酪酸梭菌预防小鼠幽门结扎型胃溃疡的机制研究

发布时间：2018-08-09 19:36

【摘要】：目的:建立小鼠幽门结扎型胃溃疡(gastric ulcer,GU)模型,观测酪酸梭菌(C.butyricum)对GU的预防作用并探讨其机制。方法:将40只ICR小鼠随机分为假手术组、模型组、奥美拉唑预防给药组和C.butyricum预防给药组。采用小鼠胃幽门结扎的方法建立GU模型,测量各组胃游离黏液量、胃液的p H、胃蛋白酶活性,并做常规HE染色观察胃组织的病理形态学变化,糖原(PAS)染色法观察胃组织糖原含量的变化,采用免疫组化检测胃黏膜组织中Bax、Bcl-2蛋白表达水平。结果:HE及PAS染色结果表明,C.butyricum预防能够显著减轻胃粘膜的损伤,其效果与奥美拉唑相当。与模型组相比,C.butyricum组胃液的p H显著升高(P0.01);胃蛋白酶活性下降(P0.05),但其效果不如奥美拉唑组(P0.01);胃游离黏液量升高(P0.01),糖原染色加深,与奥美拉唑组相比明显增多;Bax蛋白表达量降低(P0.01)但Bcl-2蛋白表达水平显著上调(P0.01)。结论:C.butyricum对小鼠幽门结扎型GU具有较好的预防作用,其机制可能与抑制胃酸分泌、胃蛋白酶活化,尤其是增加胃游离黏液的产生以及诱导bcl-2、抑制bax基因表达有关。
[Abstract]:Objective: to establish a mouse model of gastric ulcer with pyloric ligation (gastric ulcera), observe the preventive effect of Clostridium caseate (C.butyricum) on gu and explore its mechanism. Methods: forty ICR mice were randomly divided into sham operation group, model group, omeprazole preventive administration group and C.butyricum preventive administration group. The mouse gastric pyloric ligation method was used to establish the gu model. The gastric free mucus quantity, gastric pH and pepsin activity were measured in each group. The pathological changes of gastric tissue were observed by routine HE staining. The changes of glycogen content in gastric tissue were observed by (PAS) staining, and the expression of Baxfen Bcl-2 protein in gastric mucosa was detected by immunohistochemistry. Results the results of 1: he and PAS staining showed that C. butyricum could significantly reduce gastric mucosal injury, and its effect was similar to that of omeprazole. Compared with the model group, the gastric juice of C. butyricum group significantly increased (P0.01), pepsin activity decreased (P0.05), but its effect was not as good as omeprazole group (P0.01), gastric free mucus content increased (P0.01), glycogen staining deepened. Compared with omeprazole group, the expression of Bax protein decreased significantly (P0.01), but the expression of Bcl-2 protein increased significantly (P0.01). Conclusion: 1. C.butyricum has a good preventive effect on pyloric ligation type gu in mice. The mechanism may be related to inhibition of gastric acid secretion, activation of pepsin, especially increase of gastric free mucus production, induction of bcl-2 and inhibition of bax gene expression.
【作者单位】：温州医科大学基础医学院;温州医科大学第一临床学院;温州医科大学检验医学院生命科学学院;温州医科大学第二临床学院;温州医科大学附属第二医院;
【基金】：温州市科技局(No.Y20130215;No.Y20110132) 浙江省新苗计划项目(No.2014R413032)
【分类号】：R573.1

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