循环microRNA检测在大鼠溃疡性结肠炎诊断及疗效监测中的意义

发布时间：2018-08-30 12:30

【摘要】：目的探索并建立一种基于循环micro RNA的诊断大鼠溃疡性结肠炎(ulcerative colitis,UC)方法并监测其治疗后效果的临床前策略。方法第1阶段:硫酸葡聚糖(Dextran sulfate sodium,DSS)诱导大鼠UC模型。实时定量RT-PCR筛选差异性的血清micro RNA。ELISA检测血清核周抗中性粒细胞胞浆抗体(periunclear antineutrophil cytoplasmic antibodies,p ANCA)。Logistic回归法建立诊断模型Indice。受试者工作特征(receiver operating characteristic,ROC)曲线评价指标的诊断效能。第2阶段:验证指标对UC的诊断效能,并用其评价UC的疗效。结果第1阶段:大鼠UC模型建立成功。Mi R-20b、mi R-21和let-7e*在UC大鼠血清中高表达,mi R-125b-1*无明显变化。p ANCA在UC大鼠血清中高表达。诊断模型Indice=0+(2.645×mi R-20b)+(1.622×mi R21)+(0.979×let-7e*)+(0.082×p ANCA)的诊断效能强于各单项指标。第2阶段:各指标均能监测UC疗效,Indice的监测效能最强。结论循环micro RNA能稳定高效的诊断UC并监测其治疗后效果。
[Abstract]:Objective to explore and establish a method of diagnosing ulcerative colitis (ulcerative colitis,UC) based on circulating micro RNA and to monitor its effect after treatment. Methods in the first stage, dextran sulfate (Dextran sulfate sodium,DSS) was used to induce rat UC model. Real-time quantitative RT-PCR screening for differential Serum micro RNA.ELISA Detection of Serum Peripheral Anti-neutrophil Cytoplasmic Antibody (periunclear antineutrophil cytoplasmic antibodies,p ANCA) .Logistic regression to establish a Diagnostic Model Indice. The diagnostic efficacy of (receiver operating characteristic,ROC curve was evaluated. Stage 2: to verify the diagnostic efficacy of UC and evaluate the efficacy of UC. Results in the first stage, the rat UC model was established successfully. Mi R-20bmmi R-21 and let-7e* were highly expressed in the serum of UC rats. There was no significant change in the expression of Mi R-125b-1 *. P ANCA was overexpressed in the serum of UC rats. The diagnostic efficacy of Indice=0 (2.645 脳 mi R-20b) (1.622 脳 mi R21) (0.979 脳 let-7e*) (0.082 脳 p ANCA) was better than that of each single index. Stage 2: each index can monitor the curative effect of UC and Indice is the most effective. Conclusion circulating micro RNA can diagnose UC stably and efficiently and monitor its effect after treatment.
【作者单位】：重庆医科大学附属口腔医院口腔疾病与生物医学重庆市重点实验室;重庆市高校市级口腔生物医学工程重点实验室;重庆医科大学检验诊断学实验室;
【基金】：重庆市自然科学基金(CSTC2014jcyj A10055) 重庆市渝北区科委项目(渝北财教[2013])
【分类号】：R574.62

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