肝硬化和肝癌患者外周血淋巴细胞中肿瘤抑制因子EGR-1、PTEN的表达

发布时间：2018-09-09 15:11

【摘要】：目的探讨肝硬化和肝癌患者外周血淋巴细胞中肿瘤抑制因子早期生长反应因子1(EGR-1)、同源性磷酸酶-张力蛋白(PTEN)的mRNA表达水平对肝硬化、肝癌诊断和鉴别诊断的价值。方法选择收治的诊断为肝癌和肝硬化的住院患者,采用实时定量反转录-聚合酶链反应(RT-q PCR)检测患者外周血淋巴细胞中EGR-1、PTEN的mRNA表达水平;以门诊的健康体检者作为健康对照组。结果纳入患者肝癌20例(肝癌组),肝硬化22例(肝硬化组),健康对照者34例(健康对照组)。肝癌组EGR-1 mRNA表达明显低于健康对照组(△Ct:8.31±2.39 vs 9.61±1.60,P=0.018)和肝硬化组(△Ct:8.31±2.39 vs 9.68±1.81,P=0.022),而肝硬化组和健康对照组EGR-1的mRNA表达水平差异无统计学意义(P=0.890);3组PTEN的mRNA表达水平比较差异均无统计学意义(P0.05)。结论肝癌患者外周血淋巴细胞中EGR-1的mRNA表达水平降低,可能成为肝癌与肝硬化鉴别的辅助参考指标。
[Abstract]:Objective to investigate the value of mRNA expression of tumor suppressor early growth factor 1 (EGR-1) and homologous phosphatase-tension protein (PTEN) in peripheral blood lymphocytes of patients with liver cirrhosis and liver cancer in the diagnosis and differential diagnosis of liver cancer. Methods the mRNA expression of EGR-1,PTEN in peripheral blood lymphocytes was detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-q PCR) in inpatients with liver cancer and cirrhosis. Results 20 cases of liver cancer (HCC group), 22 cases of cirrhosis (cirrhosis group) and 34 cases of healthy control group (healthy control group) were included. The expression of EGR-1 mRNA in liver cancer group was significantly lower than that in healthy control group (Ct:8.31 卤2.39 vs 9.61 卤1.60 vs) and cirrhosis group (Ct:8.31 卤2.39 vs 9.68 卤1.81 vs 0.022), but there was no significant difference in mRNA expression level between liver cirrhosis group and healthy control group (P0. 890). There was no statistical significance (P0.05). Conclusion the decrease of EGR-1 mRNA expression in peripheral blood lymphocytes of patients with liver cancer may be a useful marker for differentiating liver cancer from cirrhosis.
【作者单位】：广东省汕头市中心医院消化内科;南方医科大学第一临床医学院;
【分类号】：R735.7;R575.2

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