miR-122在骨髓间充质干细胞治疗大鼠急性肝损伤中的作用

发布时间：2018-10-10 09:57

【摘要】：目的:探讨miR-122在骨髓间充质干细胞(BMSCs)治疗大鼠急性肝损伤中的作用,阐明相关作用机制。方法:密度梯度离心法分离收集雄性大鼠的BMSCs,分为转染组和对照组,其中转染组BMSCs采用脂质体介导转染miR-122mimics,对照组BMSCs未接受转染。将60只四氯化碳(CCl4)诱导的急性肝损伤大鼠随机分为肝损伤对照组(静脉注射生理盐水)、普通治疗组(静脉移植普通BMSCs)和实验治疗组(静脉移植转染miR-122的BMSCs)(n=20)。移植细胞后1、7和14d检测大鼠肝功能,肝组织行HE染色观察其病理学改变。结果:转染miR-122mimics后7d,BMSCs中白蛋白(ALB)表达水平明显上调,而甲胎蛋白(AFP)的表达水平则明显下调。移植细胞后1d,与普通治疗组比较,实验治疗组血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性差异无统计学意义(P0.05);移植细胞后7和14d时,实验治疗组大鼠血清ALT和AST活性均低于普通治疗组(P0.05);HE染色,实验治疗组大鼠肝脏充血、细胞质内空泡变性及肝细胞坏死情况明显好于普通治疗组。结论:上调BMSCs中miR-122表达水平能够促进其分化为肝样细胞,并可明显改善CCl4所致大鼠肝脏损伤。
[Abstract]:Aim: to investigate the role of miR-122 in the treatment of acute liver injury by bone marrow mesenchymal stem cell (BMSCs) in rats. Methods: BMSCs, isolated from male rats by density gradient centrifugation was divided into two groups: transfection group and control group. BMSCs in transfection group was transfected with liposome and miR-122mimics, control group BMSCs was not transfected. Sixty rats with acute liver injury induced by carbon tetrachloride (CCl4) were randomly divided into three groups: control group (intravenously injected normal saline), general treatment group (intravenous BMSCs) and experimental group (miR-122 transfected BMSCs) (nong20). The liver function was detected on the 7th and 14th day after transplantation, and the pathological changes were observed by HE staining. Results: the expression of albumin (ALB) was up-regulated and the expression of alpha-fetoprotein (AFP) was down-regulated 7 days after transfection of miR-122mimics. There was no significant difference in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities between the experimental group and the general treatment group on the 1st day after transplantation (P0.05), but at 7 and 14 days after transplantation, there was no significant difference in the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The activities of serum ALT and AST in the experimental group were lower than those in the general treatment group (P0.05). The liver hyperemia, cytosolic vacuolar degeneration and hepatocyte necrosis in the experimental group were significantly better than those in the common treatment group. Conclusion: upregulating the expression of miR-122 in BMSCs can promote its differentiation into hepatoid cells and improve the liver injury induced by CCl4 in rats.
【作者单位】：吉林医药学院附属医院普外科;第四军医大学西京医院肝胆外科;吉林大学中日联谊医院普外3科;
【基金】：国家自然科学基金资助课题(30872480)
【分类号】：R575

【参考文献】