抗纤软肝胶囊对大鼠肝纤维化肝星状细胞活化及凋亡的影响
发布时间：2018-10-13 09:32
【摘要】：目的探讨抗纤软肝胶囊对大鼠肝纤维化肝星状细胞(HSC)活化及凋亡的影响。方法将32只清洁级SD大鼠采用随机数字表法分为正常对照组和肝纤维化建模组各16只。建模组大鼠皮下注射四氯化碳(CCl_4)诱导大鼠肝纤维化,正常对照组以同样方法注射等量植物油,随后将正常对照组随机分为正常组8只,药物组8只,建模组随机分为模型组8只,治疗组8只。药物组、治疗组均给予抗纤软肝胶囊治疗,其他组同期灌服相当量的生理盐水。应用苏木素-伊红(HE)染色和Masson染色观察各组肝脏病理组织学改变;检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(ALB)、透明质酸(HA)、层黏连蛋白(LN)、Ⅳ-C型胶原(Ⅳ-C)及Ⅲ型前胶原(PC-Ⅲ)水平;RT-PCR法或流式细胞术检测各组血清体外对HSC-T6细胞中α平滑肌动蛋白(α-SMA)和转化生长因子(TGF)-β1 mRNA表达以及细胞凋亡水平的影响。结果与模型组大鼠比较,治疗组大鼠肝脏的肝纤维化程度减轻;治疗组血清ALT、AST、HA、LN、IV-C、PC-Ⅲ水平明显低于模型组,而ALB水平明显高于模型组(均P0.05);体外实验发现药物组及治疗组α-SMA和TGF-β1 mRNA的表达水平显著低于正常组及模型组(均P0.05)。流式细胞术检测结果显示,药物组及治疗组HSC-T6的凋亡水平显著高于正常组及模型组(均P0.05)。结论抗纤软肝胶囊通过参与调控HSC的活化及凋亡,可发挥对肝纤维化大鼠的治疗作用。
[Abstract]:Objective to investigate the effect of Kangxian Ruanggan capsule on (HSC) activation and apoptosis of hepatic stellate cells in rats with hepatic fibrosis. Methods 32 clean grade SD rats were randomly divided into normal control group and hepatic fibrosis model group. The model group rats were subcutaneously injected with carbon tetrachloride (CCl_4) to induce hepatic fibrosis, and the normal control group was injected with the same amount of vegetable oil in the same way. Then the normal control group was randomly divided into normal group (n = 8) and drug group (n = 8). The model group was randomly divided into model group (n = 8) and treatment group (n = 8). The drug group and the treatment group were treated with Kangxian Ruanggan capsule, while the other groups were given a considerable amount of normal saline at the same time. The histopathological changes of liver were observed by hematoxylin eosin (HE) staining and Masson staining. Serum alanine aminotransferase (ALT),) aspartate aminotransferase (AST),) albumin (ALB), (HA), laminin type 鈪，
本文编号：2268121
[Abstract]:Objective to investigate the effect of Kangxian Ruanggan capsule on (HSC) activation and apoptosis of hepatic stellate cells in rats with hepatic fibrosis. Methods 32 clean grade SD rats were randomly divided into normal control group and hepatic fibrosis model group. The model group rats were subcutaneously injected with carbon tetrachloride (CCl_4) to induce hepatic fibrosis, and the normal control group was injected with the same amount of vegetable oil in the same way. Then the normal control group was randomly divided into normal group (n = 8) and drug group (n = 8). The model group was randomly divided into model group (n = 8) and treatment group (n = 8). The drug group and the treatment group were treated with Kangxian Ruanggan capsule, while the other groups were given a considerable amount of normal saline at the same time. The histopathological changes of liver were observed by hematoxylin eosin (HE) staining and Masson staining. Serum alanine aminotransferase (ALT),) aspartate aminotransferase (AST),) albumin (ALB), (HA), laminin type 鈪，
本文编号：2268121
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