非禁食对浸水-束缚应激大鼠胃黏膜损伤的保护作用

发布时间：2018-10-22 10:53

【摘要】：目的探讨非禁食和禁食对大鼠应激性胃溃疡胃黏膜损伤及MMP-9、TIMP-2表达的影响。方法非禁食应激组不予禁食,禁食应激组于应激前24 h禁食。用浸水-束缚应激(waterimmersion and restraint stress,WIRS)建立大鼠应激性溃疡模型。应激3 h后,观察非禁食应激组和禁食应激组大鼠胃黏膜病变,对溃疡指数(UI)进行评分;组织学观察胃出血;用Western blot检测胃黏膜MMP-9、TIMP-2的蛋白表达。结果 WIRS应激3 h能成功诱导大鼠应激性胃溃疡。非禁食应激组与禁食应激组比较,应激性胃溃疡大鼠的UI后者高于前者(P0.01),黏膜层炎性细胞浸润数后者高于前者(P0.01),WIRS大鼠胃黏膜MMP-9蛋白表达后者显著高于前者(P0.01),而TIMP-2蛋白表达后者略低于前者(P0.05)。结论非禁食应激可显著减少MMP-9表达。非禁食处理减轻浸水-束缚应激大鼠胃黏膜损伤。
[Abstract]:Objective to investigate the effects of non-fasting and fasting on gastric mucosal injury and MMP-9,TIMP-2 expression in rats with stress-induced gastric ulcer. Methods No fasting was observed in the non-fasting stress group, but no fasting was observed in the non-fasting stress group 24 hours before stress. Rat stress ulcer model was established by immersion-restraint stress (waterimmersion and restraint stress,WIRS). After 3 hours of stress, gastric mucosal lesion was observed in non-fasting stress group and fasting stress group, ulcer index (UI) was scored, gastric hemorrhage was observed by histology, and MMP-9,TIMP-2 protein expression in gastric mucosa was detected by Western blot. Results WIRS stress for 3 h could successfully induce stress gastric ulcer in rats. The comparison between non-fasting stress group and fasting stress group, The UI of rats with stress gastric ulcer was higher than that of the former (P0.01), and the number of inflammatory cell infiltration in the mucosal layer was higher than that of the former (P0.01), WIRS). The latter was significantly higher than that of the former (P0.01), while the expression of TIMP-2 protein was slightly lower than that of the former (P0.05). Conclusion non-fasting stress can significantly reduce the expression of MMP-9. Non-fasting treatment alleviated gastric mucosal injury in rats with immersion-restraint stress.
【作者单位】：第三军医大学研究生管理大队;北京军区总医院消化内科;
【基金】：全军后勤科研“十二五”计划重点项目(BWS11J058)~~
【分类号】：R573.1

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