克罗恩病人肠黏膜炎性区域真菌菌群组成变化研究

发布时间：2018-11-01 21:21

【摘要】：目的 :比较克罗恩病人肠黏膜炎性与非炎性区域真菌菌群组成的差异,探讨黏膜真菌菌群变化与克罗恩病的潜在联系。方法 :手术收集7例活动性克罗恩病人肠黏膜炎性与非炎性区域组织,提取DNA,采用巢式PCR扩增18S r RNA基因片段,行变性梯度凝胶电泳,确定肠黏膜真菌菌群多样性和组成。结果 :与非炎性黏膜相比,炎性区域真菌菌群丰富度和多样性显著升高(P0.05)。肠黏膜炎性区域真菌菌群组成发生显著改变,主要表现为条件致病白色念珠菌,热带假丝酵母菌,串珠状赤霉菌,甘蓝链格孢菌和新型隐球菌的相对丰度显著增加(P0.05);而共生菌酿酒酵母菌,芽殖酵母菌,产黄青霉菌和双色蜡蘑菌显著降低(P0.05)。结论 :克罗恩病人肠黏膜炎性区域真菌菌群严重失衡,条件致病真菌定植增加可能参与了肠黏膜炎性损伤的形成。
[Abstract]:Aim: to study the relationship between mucosal fungal flora change and Crohn's disease. Methods: the intestinal mucosal inflammatory and non-inflammatory tissues were collected from 7 active Crohn patients. The 18s r RNA gene fragment was amplified by nested PCR, and the bacterial diversity and composition of intestinal mucosal fungi were determined by denaturing gradient gel electrophoresis (DGGE). Results: compared with non-inflammatory mucosa, the abundance and diversity of fungal flora in inflammatory region were significantly increased (P0.05). The composition of fungal flora in the inflammatory area of intestinal mucosa changed significantly, mainly showing that the relative abundance of Candida albicans, Candida tropicalis, Streptococcus cerevisiae, Alternaria napus and Cryptococcus neoformans increased significantly (P0.05). While symbiotic bacteria, Saccharomyces cerevisiae, Bacillus sprouts, Penicillium flavus and Tricholoma bicolor decreased significantly (P0.05). Conclusion: the fungal flora in Crohn's intestinal mucosal inflammatory region is seriously out of balance and the increased colonization of conditional pathogenic fungi may be involved in the formation of intestinal mucosal inflammatory injury.
【作者单位】：南京大学医学院附属金陵医院(解放军南京总医院)解放军普通外科研究所;南京大学医学院附属金陵医院(解放军南京总医院)呼吸内科;
【基金】：国家重点基础研究发展计划(973计划)项目(2013CB531403)
【分类号】：R574.62

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