MR定量分析兔肝脏脂肪含量与病理对照研究

发布时间：2018-11-02 07:49

【摘要】：目的以病理为金标准，探讨磁共振成像技术（正反相位DGE-MRI、质子波谱1H-MRS）在兔非酒精性脂肪肝中定量分析的价值。 材料与方法选用40只健康雄性新西兰兔，，随机分为对照组(n=15)与实验组(n=25)，对照组喂食普通饲料，实验组采用高脂饲料喂养建立非酒精性脂肪肝模型。喂养6周后，应用DGE-MRI和1H-MRS两种扫描方法，扫描结束后处死实验动物，取出肝组织作病理切片，以组织病理为金标准，分析肝脏脂肪含量（hepaticfat fraction，HFF）及脂水比（lipid water ratio，LWR）组间差异性，组间差异采用两独立样本t检验；应用ROC曲线分析HFF指数、LWR指数诊断肝细胞脂肪变（hepatic steatosis，HS）≥5%的敏感度、特异性，应用Kappa检验评价DGE-MRI、1H-MRS分析肝脏脂肪变的准确性。 结果1、建模成功率。普通饲料喂养对照组15只均无肝细胞脂肪变；高脂喂养引发兔非酒精性脂肪肝，实验组25只，发生肝细胞脂肪变20只，无肝细胞脂肪变5只，建模成功率达80%（20/25）。2、普通饲料喂养对照组与高脂喂养实验组HFF指数、LWR指数均数比较。HFF指数组间均数差异有统计学意义，P＜0.05，对照组与实验组均数分别为（8.710±1.886）%、（11.987±3.590）%。LWR指数组间均数差异有统计学意义， P＜0.05，对照组与实验组均数分别为（0.419±0.401）%、（1.684±0.995）%。3、DGE-MRI、1H-MRS诊断肝细胞脂肪变HS≥5%阈值。DGE-MRI诊断肝细胞脂肪变HS≥5%阈值9.027%，敏感度0.90，特异性0.80，AUC0.750。1H-MRS诊断肝细胞脂肪变HS≥5%阈值0.852%，敏感度1.00，特异性0.80，AUC0.860。4、DGE-MRI、1H-MRS诊断肝细胞脂肪变或脂肪肝与组织病理学诊断一致性分析。DGE-MRI Kappa值0.651，1H-MRS Kappa值0.865。两种诊断方法与组织病理诊断均有一致性，1H-MRS与组织病理诊断一致性高于DGE-MRI。 结论雄性新西兰兔高脂喂养后可成功构建非酒精性脂肪肝模型。DGE-MRI、1H-MRS在兔非酒精性脂肪肝的定量分析中有较高价值，1H-MRS诊断效能优于DGE-MRI。
[Abstract]:Objective to evaluate the value of magnetic resonance imaging (1H-MRS) in quantitative analysis of non-alcoholic fatty liver in rabbits. Materials and methods Forty healthy male New Zealand rabbits were randomly divided into two groups: control group (n = 15) and experimental group (n = 25). The control group was fed with normal diet and the experimental group was fed with high fat diet to establish a non-alcoholic fatty liver model. After 6 weeks of feeding, the experimental animals were killed by DGE-MRI and 1H-MRS scanning methods. The liver tissue was taken out for pathological section. The fat content of liver was analyzed by (hepaticfat fraction, with tissue pathology as gold standard. HFF) and lipide-water ratio (lipid water ratio,LWR) were compared with each other. Two independent samples were used to test the differences. The sensitivity and specificity of HFF index and LWR index in the diagnosis of hepatocyte steatosis (hepatic steatosis,HS) 鈮

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