MANF蛋白表达水平与肝纤维化程度的相关性研究

发布时间：2018-11-06 12:03

【摘要】：目的探讨中脑星形胶质细胞源性神经营养因子(MANF)蛋白在慢性乙型肝炎病毒(HBV)感染患者肝纤维化发生发展中的作用及其与临床特点的相关性。方法采用相对和绝对定量同位素标记(ITRAQ)蛋白质组学和免疫组化法检测肝脏穿刺组织中MANF蛋白的表达,分析其表达水平与肝脏炎症纤维化分期的关系;采用实时荧光定量PCR技术检测正常对照(NC)、乙肝病毒携带者(ASC)、慢性乙型肝炎患者(CHB)及乙肝后肝硬化患者(LC)外周血白细胞中MANF mRNA的表达水平,分析其与不同阶段慢性HBV感染者的临床病毒学和生化学指标的相关性。结果 MANF蛋白主要在肝细胞质中表达,其表达水平随着肝脏炎症及纤维化的程度加重而升高。NC组、ASC组和CHB组分别与LC组比较,MANF mRNA的表达差异均有统计学意义(P0.01)。MANF mRNA的表达水平在乙肝病毒表面抗原(HBsA g)1.5×106IU/L、(1.5×106~2.0×107)IU/L和2.0×107IU/L 3组间差异有统计学意义(P0.05);在乙肝病毒e抗原(HBeA g)阳性组与阴性组之间差异有统计学意义(P0.01);在胆红素正常组与异常组之间差异有统计学意义(P0.01)。结论 MANF蛋白可能参与了慢性HBV感染者肝纤维化的发生发展,并与其临床特点相关。
[Abstract]:Objective to investigate the role of mesencephalic astrocyte-derived neurotrophic factor (MANF) protein in the development of hepatic fibrosis in patients with chronic hepatitis B virus (HBV) infection and its correlation with clinical features. Methods relative and absolute quantitative isotope labeling (ITRAQ) proteomics and immunohistochemical method were used to detect the expression of MANF protein in liver puncture tissues, and the relationship between the expression level and the stage of hepatic inflammatory fibrosis was analyzed. The expression of MANF mRNA in peripheral blood leukocytes of patients with (ASC), chronic hepatitis B and posthepatitic cirrhosis of (LC) was detected by real-time fluorescence quantitative PCR. To analyze the correlation between the clinical virology and biochemistry of chronic HBV infection in different stages. Results MANF protein was mainly expressed in the cytoplasm of liver, and its expression level increased with the exacerbation of liver inflammation and fibrosis. NC group, ASC group and CHB group were compared with LC group, respectively. There were statistically significant differences in the expression of MANF mRNA (P0.01). MANF mRNA expression level in (HBsA g) 1.5 脳 106 IUP / L, P 0.01). There was significant difference between (1.5 脳 106) IU/L and 2.0 脳 107IU/L groups (P0.05). There was significant difference between positive and negative groups of hepatitis B virus e antigen (HBeA g) (P0.01), and between normal group of bilirubin and abnormal group (P0.01). Conclusion MANF protein may be involved in the occurrence and development of hepatic fibrosis in patients with chronic HBV infection and may be related to its clinical features.
【作者单位】：安徽医科大学第二附属医院肝病科;安徽医科大学基础医学院;
【基金】：安徽高校省级自然科学研究项目(编号:KJ2014A107)
【分类号】：R575.2

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