大鼠远端肠道病变与部分胃肠激素、胃内pH值和胃粘膜组织关系的研究

发布时间：2018-11-07 09:23

【摘要】：目的 探讨大鼠在发生肠道不完全梗阻和肠管出现溃疡性结肠炎（Ulcerativecolitis，UC）等远端肠道病变后，在发病24小时（h）和1周(w)后血清中的胃动素（motilin，MTL）和胆囊收缩素（cholecystokinin,CCK）两种胃肠激素的浓度改变情况，以及大鼠在发病后的24h和1w胃内pH值和胃粘膜的病理组织学变化。 方法 采用随机区组法将40只大鼠分为对照组（Sham组，n=10）、不完全小肠梗阻组（Small组，，n=10）、不完全结肠梗阻组（Large组，n=10）和溃疡性结肠炎组（UC组，n=10）。每组分别随机选取5只大鼠于手术后24h处死，另5只于手术后1w处死。采取心脏采血的方法抽取大鼠血液并分离提取血清，通过ELISA的方法得出全部样本的MTL和CCK的具体数值，并且在将其腹腔打开抽取胃液进行pH值测定后取大鼠的胃粘膜组织进行病理组织学分析。选用统计学中常用的SPSS17.0软件进行数据分析，对于MTL和CCK的连续变量资料使用x±s表示，运用独立样本t检验的方法对MTL和CCK的数值变化进行统计学分析，P0.05为差异有统计学意义。 结果 1.与Sham组相比，其余三组在造模24h后的MTL和CCK均明显升高，而在造模1w后两种激素均明显下降（均P0.05）； 2.Small组和Large组在24h和1w后的MTL和CCK均较UC组变化明显（均P0.05）； 3.Small组和Large组的MTL和CCK在24h和1w后比较均无明显不同（均P≥0.05）； 4.Small、Large和UC组大鼠在24h后的胃粘膜接近正常组织，而在1w后表现为轻度的炎性改变，按照慢性胃炎的评分标准其均表现为1分； 5.24h后四组两两比较，可见Small组和Large组的胃内pH值明显升高（P1=0.012，P2=0.005），而UC组与Sham组相比无明显变化（P=0.701）； 6.1w后与Sham组相比，Small、Large和UC组的胃内pH值均明显升高（均P=0.000）,而两两比较发现三组之间的pH值无明显差异（P1=0.121，P2=0.211，P3=0.433）。 结论 大鼠远端肠道病变如Small、Large和UC组的MTL和CCK两种胃肠激素在发病初期明显升高，一周后则下降。而当大鼠远端肠道出现病变后其胃内pH值会升高并且胃粘膜组织可能会出现一定程度的炎性改变。由此可推测远端肠道病变不仅可引起局部发病部位的病理变化，而且还可能对上消化道甚至全身形成一定的影响。通过本次实验的实验结果可以推测，胃肠激素与远端肠道病变的不同病程阶段密切相关，提醒广大临床工作者在对远端肠道病变时，应参考患者胃肠激素水平的情况，从而对远端肠道病变病程的评估提供一定的依据。并且提示，当机体发生远端肠道病变时不仅需要给予对症治疗，而且应当在胃肠激素方面给予相应的调整，还要关注上消化道甚至是全身的情况，以便做出及时的处理。
[Abstract]:Objective to investigate the serum motilin (motilin,) levels of ulcerative colitis (Ulcerativecolitis,UC) in rats after 24 hours of (h) and 1 week of (w) after the onset of incomplete intestinal obstruction and ulcerative colitis (Ulcerativecolitis,UC). The changes of gastrointestinal hormones (MTL) and cholecystokinin (cholecystokinin,CCK), and the changes of gastric pH and gastric mucosal histopathology in rats at 24 h and 1 week after the onset of the disease. Methods Forty rats were randomly divided into control group (Sham group, n = 10), incomplete small bowel obstruction group (Small group, n = 10), incomplete colonic obstruction group (Large group, n = 10) and ulcerative colitis group (UC group, n = 10). In each group, 5 rats were randomly selected to be killed 24 hours after operation and 5 rats were killed at 1 week after operation. The rat blood was taken from the heart and the serum was isolated. The specific values of MTL and CCK of all the samples were obtained by the method of ELISA. The gastric mucosal tissue of the rats was analyzed by histopathology after the gastric juice was extracted from the abdominal cavity for the determination of pH value. The data were analyzed by using SPSS17.0 software, the data of MTL and CCK were expressed by x 卤s, and the numerical changes of MTL and CCK were analyzed by independent sample t-test. P0.05 for the difference was statistically significant. Result 1. Compared with the Sham group, the MTL and CCK of the other three groups increased significantly after 24 hours of modeling, but the two hormones decreased significantly after 1 week (P0.05). The changes of MTL and CCK in 2.Small group and Large group were significantly higher than that in UC group after 24 h and 1 week (P0.05), MTL and CCK in 3.Small group and Large group were not significantly different after 24 h and 1 week (all P > 0 05). 4. The gastric mucosa of small large group and UC group was close to normal tissue after 24 hours, but it showed mild inflammatory change after 1 week, according to the score of chronic gastritis, all rats showed 1 score. 5.After 24 hours, compared with the four groups, the intragastric pH value of Small group and Large group was significantly higher (P10.012 P2P 0.005), but that of UC group was not significantly different from that of Sham group (P0. 701). Compared with Sham group, the intragastric pH value in Small,Large and UC groups was significantly higher (P < 0. 000), but there was no significant difference in pH value between the three groups (P 1: 0. 121 P 2 0. 211 P 30.33). Conclusion Gastrointestinal hormones MTL and CCK in distal intestinal lesions such as Small,Large and UC increased at the beginning of the disease and decreased one week later. However, the pH value in the stomach increased and the gastric mucosal tissue appeared inflammatory changes after the distal intestinal lesions in rats. It can be inferred that distal intestinal lesions can not only cause pathological changes in local lesions, but also may affect the formation of upper digestive tract and even the whole body. According to the experimental results of this experiment, it can be inferred that gastrointestinal hormones are closely related to different stages of distal intestinal lesions. The majority of clinical workers should refer to the level of gastrointestinal hormones in patients with distal intestinal lesions. Therefore, it provides some basis for the evaluation of the course of distal intestinal lesions. It is suggested that when the body has distal intestinal lesions, it should not only be treated with symptomatic treatment, but also should be adjusted in terms of gastrointestinal hormones, and should pay attention to the situation of upper digestive tract and even the whole body so that timely treatment can be made.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R57

【参考文献】

相关期刊论文 前10条

1 梁荣新 ,蔡联英 ,郑琴芳 ,张法灿;肠易激综合征结肠动力与胃肠激素的相关性研究[J];广西医学;2005年04期

2 徐斌;周振理;;肠梗阻病因及治疗分析[J];吉林医学;2012年14期

3 杜娟,王秀琴;胃泌素的生物活性及其信号传导通路[J];解剖科学进展;2003年04期

4 陈代陆;胆囊收缩素对消化道运动调节作用的研究进展[J];临床消化病杂志;2002年04期

5 田志颖;冯丽英;王丽华;;炎症性肠病和肠易激综合征相关性研究进展[J];临床合理用药杂志;2011年14期

6 黄穗平,李叶,罗云坚,尚文,余绍源;功能性消化不良中医证型与胃肠动力及激素的相关性[J];广州中医药大学学报;2002年04期

7 崔英;;影像学检查在胃肠功能性疾病中的应用价值[J];中国实用内科杂志;2007年08期

8 李晓波,刘文忠,戈之铮,萧树东;报警症状在胃肠道疾病诊断中的作用[J];胃肠病学;2005年04期

9 王承党;;重视黏膜低度炎症在肠易激综合征发病机制中的作用[J];胃肠病学;2008年09期

10 陈文科;邹益友;李富军;罗丹;;肠易激综合征精神心理因素、肠黏膜肥大细胞及5-羟色胺的变化[J];世界华人消化杂志;2007年01期

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