构建急进高海拔地区急性应激性胃肠道损伤大鼠模型

发布时间：2018-11-08 18:48

【摘要】：背景:前急进高海拔地区胃肠道急性应激损伤的模型建立鲜有报道,建立稳定的急进高海拔肠应激性损伤模型成为当前研究急进高海拔应激性胃肠损伤及相关疾病的前提。目的:建立急进高海拔引起的急性应激性肠缺血再灌注大鼠模型。方法:将雄性Wistar大鼠24只按随机数字表法分为3组,高海拔下限正常对照组、高海拔假手术组和高海拔缺血再灌注组各8只。大鼠急进海拔地区,缺血再灌注组以血管夹夹闭肠系膜上动脉根部完全阻断血流60 min,之后松开动脉血管夹,恢复肠道血流形成再灌注,时间为60 min。假手术组仅分离肠系膜上动脉不作夹闭。正常对照组不作任何处理。大鼠再灌注60 min后,以化学发光法检测肌酸激酶同工酶、谷草转氨酶、谷丙转氨酶、尿素氮、肌酐水平,并行病理组织学观察分析。结果与结论:急进高海拔并行肠缺血再灌注对大鼠肝、肾、心功能均有不同程度的损伤,肠黏膜出血,腺体断裂甚至发生溶解,黏膜层和黏膜固有层有大量炎性细胞浸润。提示采用急进高海拔地区后动脉夹夹闭肠系膜上动脉的方法制备急进高海拔地区急性应激性胃肠道损伤的大鼠模型病情相对稳定,方法简单,适于进行急进高海拔地区急性应激性胃肠道损伤的防治研究。
[Abstract]:Background: there are few reports on the establishment of models of acute gastrointestinal tract stress injury at high altitude. Establishing a stable model of acute gastrointestinal stress injury at high altitude becomes a prerequisite for the study of acute gastrointestinal injury and related diseases at high altitude. Aim: to establish a rat model of acute stress intestinal ischemia reperfusion induced by rapid advance at high altitude. Methods: Twenty-four male Wistar rats were randomly divided into three groups: normal control group with high altitude lower limit, sham-operation group with high altitude and ischemia reperfusion group with high altitude. In the ischemia reperfusion group, the blood flow of the superior mesenteric artery was completely blocked for 60 min, then the artery clamp was released, and the intestinal blood flow was restored to reperfusion for 60 min.. In the sham operation group, the superior mesenteric artery was not clipped. No treatment was given in the normal control group. After reperfusion for 60 min, the levels of creatine kinase isoenzyme, glutamic oxaloacetic transaminase, alanine aminotransferase, urea nitrogen and creatinine were detected by chemiluminescence assay. Results and conclusion: the liver, kidney and heart function of rats were damaged in different degree by acute high altitude and intestinal ischemia-reperfusion, intestinal mucosal hemorrhage, glandular rupture or even dissolution, and a large number of inflammatory cells infiltrated in mucosal layer and lamina propria. The results suggested that the rat model of acute stress gastrointestinal injury was relatively stable and the method was simple by clamping the superior mesenteric artery at high altitude. It is suitable for the prevention and treatment of acute stress gastrointestinal injury at high altitude.
【作者单位】：解放军兰州军区兰州总医院医学检验中心;
【基金】：兰州军区后勤科研项目(CLZ11J09),课题名称:高海拔地区军事应激致胃肠损伤的基因防治药物实验研究甘肃省杰出青年基金项目(206020302),课题名称:自噬在SPKH保护缺氧肠应激损伤中的作用及其分子调控机制~~
【分类号】：R57;R-332

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