UC患者PBMC中TLR4、NF-κB水平的变化及其临床意义

发布时间：2018-11-22 19:45

【摘要】：目的探讨溃疡性结肠炎(UC)患者外周血单核细胞(PBMC)中TOLL样受体(TLR)4、NF-κB水平的变化及在UC发病中的可能机制。方法选取武警浙江省总队医院嘉兴医院消化科2014年1月至2015年1月收治的UC患者30例作为观察组,另选取该院体检科健康体检者10例作为对照组。采用流式细胞仪检测外周血CD14+单核细胞表面TLR4阳性表达率,RTPCR检测TLR4、髓样分化因子88(MyD88)、NF-κB(P65)mRNA表达水平,Western blot检测TLR4、MyD88、NF-κB(P65)蛋白水平。结果观察组外周血CD14~+单核细胞表面TLR4阳性表达率明显高于对照组(P0.05);对照组TLR4、MyD88、NF-κB(P65)mRNA及蛋白水平明显低于观察组(P0.01);TLR4、MyD88、NF-κB(P65)蛋白水平与UC严重程度呈正相关。结论 UC患者PBMC中TLR4、NF-κB明显增高,临床可通过检测TLR4、NF-κB水平判断UC的发展程度。
[Abstract]:Objective to investigate the changes of TOLL like receptor (TLR) 4 (NF- 魏 B) in peripheral blood monocyte (PBMC) in patients with ulcerative colitis (UC) and its possible mechanism in the pathogenesis of UC. Methods from January 2014 to January 2015, 30 patients with UC were selected from Jiaxing Hospital of Armed Police of Zhejiang Province as observation group, and 10 cases of healthy examination of Department of physical examination were selected as control group. The positive expression rate of TLR4 on peripheral CD14 monocytes was detected by flow cytometry. The expression of TLR4, myeloid differentiation factor 88 (MyD88) and NF- 魏 B (p65) mRNA were detected by RTPCR and TLR4,MyD88,NF- 魏 B (p65) protein by, Western blot. Results the positive expression rate of TLR4 on CD14~ monocytes in the observation group was significantly higher than that in the control group (P0.05), and the TLR4,MyD88,NF- 魏 B (p65) mRNA and protein levels in the control group were significantly lower than those in the observation group (P0.01). The level of TLR4,MyD88,NF- 魏 B (p65) protein was positively correlated with the severity of UC. Conclusion the level of TLR4,NF- 魏 B in PBMC of UC patients is significantly higher than that of PBMC. The development of UC can be judged by detecting TLR4,NF- 魏 B level in clinic.
【作者单位】：武警浙江省总队医院嘉兴医院消化内科;武警浙江省总队医院嘉兴医院放射科;浙江省嘉兴市妇幼保健医院放射科;
【分类号】：R574.62

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