感染后肠易激综合征小鼠肠黏膜和血中细胞因子的表达变化

发布时间：2018-11-28 10:00

【摘要】：【目的】观察感染后肠易激综合征小鼠不同肠段和外周血中IFN-γ、IL-17、IL-10的表达及以上细胞因子的改变对肠道动力和内脏敏感性的影响。【方法】40只雌性C57BL\6小鼠随机分为PI-IBS组和对照组,每组20只。PI-IBS组小鼠用0.2 m L含350条旋毛虫幼虫的生理盐水悬液灌胃,对照组给予等体积的生理盐水。分别于感染后第2、4、8周,取末端回肠和近端结肠进行组织病理学检测。于感染后第8周通过检测小鼠腹壁撤退反射(AWR)评分评估小鼠对结直肠扩张的内脏敏感性变化,检测肠道传输时间(ITT)和粪便含水百分数评估小鼠肠道动力的改变;采用ELISA测定十二指肠、空肠、回肠、结肠和外周血中IFN-γ、IL-17和IL-10水平的改变。【结果】感染后第8周,PI-IBS小鼠肠道急性炎症完全恢复至正常;在结直肠扩张容量为0.35和0.5 m L时,模型组AWR评分均显著高于对照组(P0.01);PI-IBS小鼠肠道传输时间缩短、每2 h粪便含水百分数增高,较正常对照组具有显著性差异(P0.01);ELISA结果显示:与对照组相比,PI-IBS组小鼠的IFN-γ和IL-17水平在十二指肠、回肠和外周血中表达升高(P0.05);而IL-10水平在空肠、回肠、结肠以及外周血中的表达显著降低(P0.05)。【结论】感染后肠易激综合征小鼠不同肠段组织中细胞因子表达水平存在差异,外周血与部分肠段组织中细胞因子的表达变化呈同步化改变。细胞因子的改变可能是PI-IBS内脏敏感性增高和肠道运动功能紊乱的机制之一。
[Abstract]:[objective] to observe IFN- 纬, IL-17, in different intestinal segments and peripheral blood of mice with irritable bowel syndrome after infection. Effects of IL-10 expression and above cytokines on intestinal motility and visceral sensitivity. [methods] Forty female C57BL\ 6 mice were randomly divided into PI-IBS group and control group. 20 mice in each group were fed with 0.2 mL saline suspension containing 350 Trichinella spiralis larvae in PI-IBS group, and the control group was given the same volume of normal saline. The terminal ileum and proximal colon were taken for histopathological examination at the 2nd week and 4th week after infection. At the 8th week after infection, the visceral sensitivity of mice to colorectal dilatation, intestinal transit time (ITT) and fecal water content were evaluated by measuring the abdominal wall withdrawal reflex (AWR) score, and the changes of intestinal motility were evaluated. The levels of IFN- 纬, IL-17 and IL-10 in duodenum, jejunum, ileum, colon and peripheral blood were measured by ELISA. When colorectal dilatation volume was 0.35 and 0.5 mL, the AWR score of model group was significantly higher than that of control group (P0.01). The intestinal transit time of PI-IBS mice was shortened and the percentage of fecal water was increased every 2 hours, which was significantly different from that of normal control group (P0.01). ELISA results showed that the levels of IFN- 纬 and IL-17 in the duodenum, ileum and peripheral blood of the PI-IBS group were higher than those of the control group (P0.05). The expression of IL-10 was significantly decreased in jejunum, ileum, colon and peripheral blood (P0.05). [conclusion] the expression of cytokines in different intestinal segments of mice with irritable bowel syndrome after infection was different. The changes of cytokine expression in peripheral blood and some intestinal segments were synchronized. The change of cytokines may be one of the mechanisms of increased visceral sensitivity and intestinal motor dysfunction in PI-IBS.
【作者单位】：重庆医科大学附属第一医院消化内科;四川省达州市中心医院消化内科;海南省人民医院消化内科;
【基金】：国家自然科学基金(81160057) 海南省国际合作项目
【分类号】：R574.4

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