乳酸链球菌素对大肠杆菌所致腹泻小鼠的脑-肠轴中单胺类神经递质的影响

发布时间：2018-12-13 06:27

【摘要】：旨在研究乳酸链球菌素(nisin)对应激性腹泻导致的肠道菌群与脑-肠轴功能之间的相关性,进一步揭示nisin对单胺类神经递质的调控机制。通过注入3种血清型大肠杆菌混合菌悬液(1∶1,1.0×109 CFU·mL-1)制备小鼠腹泻模型,并用nisin和蒙药复方进行治疗;在造模后0、72h,ELISA检测5-HT、DA和NE水平,以及平板涂布法将盲肠内容物分别10倍稀释成8个梯度涂布于相应培养基,计算菌落数。结果表明,(1)在脑组织中,造模后72h时nisin、传统蒙药复方、成分复方、抗生素组中5-HT分别升高到0h时的108.60%、124.04%、117.47%、115.57%;并且使十二指肠中5-HT分别增加到0h时的289.18%、200.30%、271.59%,nisin组显著高于其他各组(P0.05);造模后除空肠外,72h与0h相比,nisin和传统蒙药复方对小鼠血清、脑、十二指肠、回肠以及结肠中5-HT都有显著提升作用(P0.05);(2)造模后72h时,nisin、蒙药复方、成分复方及抗生素可提高正常小鼠脑、肠中多巴胺,并以回肠中增加最多,且差异显著(P0.05);(3)在脑组织中0h与72h时NE的浓度,传统蒙药复方、nisin分别升高到115.62%和106.17%,且均与空白、阴性对照组相比差异显著(P0.05);在十二指肠、回肠中,nisin组72h比0h升高了170.97%、121.74%,尤其十二指肠明显高于其他各组且差异显著(P0.05);(4)nisin、有效成分复方和传统蒙药复方可显著提高感染E.coli小鼠肠道内有益菌乳酸菌和双歧杆菌数量,显著降低有害菌肠杆菌和肠球菌数量(P0.05)。可见,nisin和蒙药复方均显著提高细菌感染小鼠血液、脑和各肠段中5-HT、NE含量,尤以十二指肠突出,nisin和传统蒙药能打破肠道中5-HT的平衡,并伴随着肠道各段、血液中5-HT的升高脑组织中5-HT含量也增加。
[Abstract]:The aim of this study was to investigate the relationship between the intestinal flora induced by stress diarrhea and the brain-intestinal axis function of nisin (nisin), and to further reveal the regulatory mechanism of nisin on monoamine neurotransmitters. The diarrhea model of mice was established by injecting three kinds of serotype Escherichia coli suspension (1: 11.0 脳 10 ~ 9 CFU mL-1) and treated with nisin and Mongolian medicine compound. The levels of 5-HTDA and NE were detected by Elisa at 0 ~ 72 h after modeling, and the contents of cecum were diluted 10 times into 8 gradient media by plate coating, and the colony number was calculated. The results showed that: (1) in brain tissue, the 5-HT in nisin, traditional Mongolian medicine compound, compound composition and antibiotic group increased to 108.60h and 124.04at 0 h, respectively. And the 5-HT in duodenum increased to 289.18% at 0 h, which was significantly higher in the control group than in the other groups (P0.05). In addition to jejunum, compared with 0 h after 72 h, nisin and traditional Mongolian medicine compound could significantly enhance 5-HT in serum, brain, duodenum, ileum and colon of mice (P0.05). (2) at 72 h after modeling, nisin, Mongolian medicine compound, composition compound and antibiotics could increase dopamine in brain and intestine of normal mice, and the increase was the most in ileum (P0.05); (3) the concentration of NE in brain tissue at 0 h and 72 h, the traditional Mongolian medicine compound and nisin increased to 115.62% and 106.17% respectively, which were significantly different from the blank group (P0.05); In duodenum and ileum, nisin group increased 170.97% 121.74 hours than 0 h, especially in duodenum significantly higher than other groups (P0.05). (4) nisin, active component compound and traditional Mongolian medicine compound could significantly increase the number of beneficial bacteria lactic acid bacteria and bifidobacterium in intestinal tract of E.coli mice, and significantly reduce the number of harmful bacteria Enterobacter and Enterococcus (P0.05). It can be seen that both nisin and Mongolian medicine compound can significantly increase the contents of 5-HT NE in blood, brain and various intestinal segments of mice infected by bacteria, especially in duodenal herniation. Nisin and traditional Mongolian medicine can break the balance of 5-HT in intestine and accompany with each segment of intestinal tract. The increase of 5-HT in blood also increased the content of 5-HT in brain tissue.
【作者单位】：内蒙古农业大学兽医学院;内蒙古农业大学动物科学学院;
【基金】：国家自然科学基金项目(31260590) 国家科技支撑项目(2015BAD29B06)
【分类号】：R574.62

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