前列腺干细胞抗原基因和磷脂酶CE1基因多态性与老年幽门螺旋杆菌感染胃溃疡的易感性及对临床预后的影响

发布时间：2018-12-16 07:55

【摘要】：目的探讨前列腺干细胞抗原基因(PSCA)和磷脂酶(PL)CE1基因多态性与老年幽门螺旋杆菌(Hp)感染胃溃疡的易感性及对临床预后的影响。方法采用病例-对照研究设计,选取130例胃溃疡并且Hp检查为阳性的老年患者作为病例组,另外按性别和年龄匹配的胃溃疡并且Hp检查为阴性的老年患者作为对照组。所有患者治疗前抽取外周静脉血并提取DNA,设计PSCA(rs2294008)和PLCE1(rs11599672)聚合酶链反应(PCR)引物进行PCR扩增,扩增产物采用DNA测序分析PSCA和PLCE1基因类型,采用Logistic回归计算校正相对危险度(OR)和95%置信区间(95%CI)评价PSCA和PLCE1基因多态性与老年Hp感染胃溃疡的易感性。结果 1两组年龄、性别、病程、病变部位差异均无统计学意义(P0.05);2与rs2294008基因型CC比较,基因型为CT、TT和CT/TT的患者Hp感染危险性降低(P0.01);3与PLCE1 rs11599672基因型AA比较,基因型为AC、CC和AC/CC的患者Hp感染危险性降低(P0.05);4不同PSCA多态位点rs2294008和LCEl多态位点rs11599672患者的Hp根除率和溃疡愈合率差异具有统计意义(P0.01)。结论 PSCA rs2294008基因型CC和PLCE1 rs11599672基因型AA可增加Hp感染胃溃疡易感性,并且显著降低临床预后。
[Abstract]:Objective to investigate the relationship between polymorphisms of prostate stem cell antigen (PSCA) and phospholipase (PL) CE1) genes and the susceptibility to gastric ulcer infection by Helicobacter pylori (Hp) in the elderly and its influence on clinical prognosis. Methods A case-control study was carried out in which 130 patients with gastric ulcer and positive Hp were selected as the case group, and the patients with gastric ulcer matched by sex and age with negative Hp were selected as the control group. Peripheral venous blood was extracted from all patients before treatment and DNA, was extracted to design PSCA (rs2294008) and PLCE1 (rs11599672) polymerase chain reaction (PCR) primers for PCR amplification. PSCA and PLCE1 gene types were analyzed by DNA sequencing. Corrected relative risk (OR) and 95% confidence interval (95%CI) were calculated by Logistic regression to evaluate the susceptibility of PSCA and PLCE1 gene polymorphisms to gastric ulcer infection in elderly Hp patients. Results (1) there was no significant difference in age, sex, course of disease and lesion site between the two groups (P0.05), 2 compared with rs2294008 genotype CC, the risk of Hp infection in patients with CT,TT and CT/TT genotypes was lower (P0.01). 3Compared with PLCE1 rs11599672 genotype AA, the risk of Hp infection in patients with genotype AC,CC and AC/CC was lower (P0.05). 4There were significant differences in Hp eradication rate and ulcer healing rate between rs2294008 and LCEl polymorphic rs11599672 patients with different PSCA polymorphic loci (P0.01). Conclusion PSCA rs2294008 genotype CC and PLCE1 rs11599672 genotype AA can increase the susceptibility of Hp to gastric ulcer infection and significantly reduce the clinical prognosis.
【作者单位】：聊城市人民医院;聊城市复退军人医院;
【基金】：山东省自然科学基金联合专项(No.ZR2014HL019)
【分类号】：R573

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