结肠炎小鼠外周血和结肠上皮组织中Gal-9的表达

发布时间：2019-01-13 07:41

【摘要】：目的:探讨半乳糖凝集素-9(Gal-9)与炎症性结肠炎(IBP)的关系。方法:60只雄性Balb/c小鼠分为4组,每组15只。对照组:自由饮食。三硝基苯磺酸钠(TNBS)组:实验第1、8、15天分别用2.0、2.5和3.0 mg TNBS加体积分数50%的乙醇溶液100μL灌肠,建立小鼠结肠炎模型。TNBS+鞭毛蛋白(CBir1)组:同上造模,并于实验第7、9、12、15天小鼠腹腔注射100μg/L的CBir1 100μL。TNBS+白细胞介素-17(IL-17)组:造模后,小鼠腹腔注射IL-17 500 ng,每周2次。第22天处死小鼠,ELISA方法检测外周血Gal-9含量,免疫组化法检测结肠上皮组织中Gal-9蛋白的表达。结果:各组小鼠血清和结肠上皮组织中Gal-9的表达差异有统计学意义(F/χ2=22.669和25.179,P均0.001),TNBS组低于对照组,TNBS+CBir1组和TNBS+IL-17组低于TNBS组(P均0.001)。结论:Gal-9可用来评估结肠炎的严重程度。
[Abstract]:Objective: to investigate the relationship between galactose agglutinin 9 (Gal-9) and inflammatory colitis (IBP). Methods: 60 male Balb/c mice were divided into 4 groups with 15 mice in each group. Control group: free diet. Sodium trinitrobenzenesulfonate (TNBS) group: mice colitis model was established by enema with 2.5 and 3.0 mg TNBS ethanol solution of 50% volume fraction on the 15th day of the experiment. TNBS flagellin (CBir1) group: same as above. The mice were injected with 100 渭 g / L CBir1 100 渭 L.TNBS interleukin-17 (IL-17) intraperitoneally on the 15th day of the experiment. After the model was established, IL-17 500 ng, was injected twice a week. On the 22nd day, the mice were killed, the Gal-9 content in peripheral blood was detected by ELISA method, and the expression of Gal-9 protein in colonic epithelium was detected by immunohistochemical method. Results: the expression of Gal-9 in serum and colonic epithelium of each group was significantly lower than that in control group (F / 蠂 2 22.669 and 25.179 P 0.001), TNBS, P < 0.05). TNBS CBir1 group and TNBS IL-17 group were lower than TNBS group (P 0.001). Conclusion: Gal-9 can be used to evaluate the severity of colitis.
【作者单位】：郑州大学第一附属医院消化内科;
【基金】：国家自然科学基金面上项目81070288,81270452 河南省医学科技学术带头人出国培训计划基金资助项目201201013
【分类号】：R574.62

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