对胃粘膜损伤有辅助保护功能的评价试验中的病理学方法的探讨与建议

发布时间：2019-01-19 19:09

【摘要】：目的建立科学的大体病理学检查和组织病理学诊断的评分原则、更合理的评分标准以及合理的统计学方法,为保健食品对胃粘膜损伤有辅助保护作用提供更为科学、可行的评价方法。方法通过对大鼠胃黏膜急性损伤酒精模型的全面分析,针对现行方法中评价方法的缺点,根据病变特点及病理学检查的原则和半定量分析方法,以及相应的统计学方法等信息化处理,进行方法学探讨。结果大体病理学检查按照病变所占面积以及在整个胃粘膜所占的比例;组织病理学诊断根据病变累及粘膜层的深度作为判定粘膜受损严重程度的主要依据,其他各种病变作为参考因素。分别分为未见异常(0分)、轻度病变(1分)、中度病变(2分)和重度病变(3分)。统计学方法采用Ridit统计学方法分析病理学半定量的结果。结论从大体病理学、组织病理学的评价方法以及数据处理方法和结果判定等方面,提出了对胃粘膜损伤有辅助保护功能的动物实验相对更为科学、可行的评价方法的建议。
[Abstract]:Objective to establish a scientific scoring principle of gross pathology and histopathological diagnosis, a more reasonable scoring standard and a reasonable statistical method, so as to provide a more scientific method for the auxiliary protective effect of health food on gastric mucosal injury. Feasible evaluation method. Methods based on the comprehensive analysis of the acute gastric mucosal injury alcohol model in rats, according to the characteristics of pathological changes and the principles of pathological examination and semi-quantitative analysis, aiming at the shortcomings of the present methods, the evaluation methods were used. And the corresponding statistical methods and other information processing, methodological discussion. Results the gross pathological examination according to the lesion area and the proportion of the whole gastric mucosa; Histopathological diagnosis was based on the depth of the involvement of the mucosal layer as the main basis for judging the severity of mucosal damage and other lesions as reference factors. There were no abnormality (0), mild lesion (1), moderate lesion (2) and severe lesion (3). Ridit statistical method was used to analyze the semi-quantitative results of pathology. Conclusion from the aspects of gross pathology, evaluation method of histopathology, data processing method and result judgment, this paper puts forward some scientific and feasible evaluation methods for gastric mucosal injury.
【作者单位】：深圳市药品检验所深圳药品质量标准研究重点实验室;中兴通讯股份有限公司;
【分类号】：R573;TS218

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