利用X射线相衬CT技术观察胆管结扎诱导肝纤维化微脉管的变化

发布时间：2019-01-22 19:34

【摘要】：目的肝纤维化是慢性肝病向肝硬化发展的必然病理过程,本研究拟探讨X射线相衬CT(PCCT)技术评估胆管结扎诱导肝纤维化中微脉管变化的价值。材料与方法胆管结扎诱导大鼠肝纤维化组和对照组肝脏组织各5例,成像实验在上海同步辐射装置的BL13W1线站进行,成像后样品进行天狼星红染色和CK19免疫组织化学染色。实验利用PCCT技术重建肝脏组织中的三维(3D)微脉管结构,并分析微脉管密度与纤维面积比之间的相关性。结果 PCCT技术清晰地展示了肝脏中的血管、扩张胆管和增生胆管等脉管结构。与对照组相比,肝纤维化组3D微脉管密度和纤维面积比均明显增大(P0.01),并且两者呈正相关(r=0.812,P0.01)。结论 PCCT成像能够清晰展示肝纤维化组织微脉管结构的3D特征,并且3D微脉管密度与纤维面积比具有高度相关性,表明在肝纤维化发展中,微脉管的变化与纤维化程度密切相关,这为利用微脉管的变化评价纤维化疾病的发展提供了一种新思路。
[Abstract]:Objective liver fibrosis is a necessary pathological process in the development of chronic liver disease to cirrhosis. The purpose of this study was to evaluate the value of CT (PCCT) technique in assessing the microvascular changes in hepatic fibrosis induced by bile duct ligation. Materials and methods five cases of hepatic fibrosis induced by ligation of bile duct and 5 cases of control group were performed at BL13W1 line station of Shanghai Synchrotron radiation device. The samples were stained with Sirius red and CK19 immunohistochemical staining after imaging. Three-dimensional (3D) microvascular structures in liver tissue were reconstructed by PCCT technique, and the correlation between microvascular density and fiber area ratio was analyzed. Results the vascular structures of liver, dilated bile duct and proliferative bile duct were clearly demonstrated by PCCT technique. Compared with the control group, 3D microvascular density and fiber area ratio in the hepatic fibrosis group were significantly increased (P0.01), and there was a positive correlation between the two groups (r = 0.812, P0.01). Conclusion PCCT imaging can clearly display the 3D characteristics of microvascular structure in liver fibrosis tissue, and the density of 3D microvascular is highly correlated with the fiber area ratio, which indicates that the changes of microvascular structure are closely related to the degree of fibrosis in the development of liver fibrosis. This provides a new way to evaluate the development of fibrosis disease by using microvascular changes.
【作者单位】：天津医科大学生物医学工程与技术学院;首都医科大学附属北京友谊医院;
【基金】：国家自然科学基金(81371549,81671683,81670545) 天津市自然科学基金(16JCYBJC 28600) 中国肝炎防治基金会-王宝恩肝纤维化研究基金资助课题(CFHPC20131033)
【分类号】：R575.2

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