自身免疫性肝病患者外周血Th17与Treg细胞及其相关因子表达的研究
发布时间：2019-01-24 21:14
【摘要】：目的研究自身免疫性肝病（AILD）患者外周血中辅助性T细胞17（Th17）、调节性T细胞（Treg细胞）及其细胞因子白细胞介素-17（IL-17）、转化生长因子-β（TGF-β）的表达情况，探讨Th17与Treg细胞在AILD发病机制中的作用及其临床意义。方法选择46例AILD患者,其中28例自身免疫性肝炎患者（AIH组），18例原发性胆汁性肝硬化患者（PBC组）和20例健康人（对照组）做为研究对象，使用流式细胞术对AIH组、PBC组及对照组的外周血单个核细胞（PBMCs）中的Treg及Th17细胞的比例进行检测；应用酶联免疫吸附法（ELISA）检测血清中Th17的相关因子IL-17和Treg的相关因子TGF-β含量。测得数据采用SPSS19.0统计软件进行分析。结果AIH组和PBC组患者PBMCs中Th17细胞比例高于对照组（P＜0.01）,Treg细胞显著低于对照组（P0.05）；AIH组和PBC组患者IL-17的含量较对照组升高（P 0.05），，血清TGF-β的含量较对照组降低（P 0.01）。结论在AILD患者中，Th17、Treg细胞的变化可能在AILD发病机制中发挥重要作用，其变化可能是引起机体免疫系统平衡倾向促炎方向而导致AILD的发病的机制之一；对Th17/Treg的观察可用于AILD疾病进展情况监测及疗效观察；IL-17和TGF-β与AILD的相关性较强，二者结合可提高诊断AILD的敏感性。
[Abstract]:Objective to study the peripheral blood helper T cell 17 (Th17), regulatory T cell (Treg cell) and its cytokine interleukin-17 (IL-17) in patients with autoimmune liver disease (AILD). The expression of transforming growth factor- 尾 (TGF- 尾) and the role of Th17 and Treg cells in the pathogenesis of AILD and its clinical significance were investigated. Methods Forty-six patients with AILD, including 28 patients with autoimmune hepatitis (AIH group), 18 patients with primary biliary cirrhosis (PBC group) and 20 healthy people (control group), were selected as subjects. Flow cytometry was used to treat AIH group. The proportion of Treg and Th17 cells in (PBMCs) of peripheral blood mononuclear cells in PBC group and control group were detected. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of Th17 related factor IL-17 and Treg related factor TGF- 尾 in serum. The measured data were analyzed by SPSS19.0 software. Results the percentage of Th17 cells in PBMCs in AIH group and PBC group was higher than that in control group (P < 0. 01). The percentage of Th17 cells in PBMCs was significantly lower than that in control group (P < 0. 01). The content of IL-17 in AIH group and PBC group was higher than that in control group (P0. 05), and the content of serum TGF- 尾 was lower than that in control group (P0. 01). Conclusion in AILD patients, the changes of Th17,Treg cells may play an important role in the pathogenesis of AILD, which may be one of the mechanisms that lead to the pro-inflammatory direction of the immune system and lead to the pathogenesis of AILD. The observation of Th17/Treg can be used to monitor the disease progression and observe the curative effect of AILD, IL-17 and TGF- 尾 have strong correlation with AILD, and the combination of IL-17 and TGF- 尾 can improve the sensitivity of AILD diagnosis.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R575
本文编号：2414868
[Abstract]:Objective to study the peripheral blood helper T cell 17 (Th17), regulatory T cell (Treg cell) and its cytokine interleukin-17 (IL-17) in patients with autoimmune liver disease (AILD). The expression of transforming growth factor- 尾 (TGF- 尾) and the role of Th17 and Treg cells in the pathogenesis of AILD and its clinical significance were investigated. Methods Forty-six patients with AILD, including 28 patients with autoimmune hepatitis (AIH group), 18 patients with primary biliary cirrhosis (PBC group) and 20 healthy people (control group), were selected as subjects. Flow cytometry was used to treat AIH group. The proportion of Treg and Th17 cells in (PBMCs) of peripheral blood mononuclear cells in PBC group and control group were detected. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of Th17 related factor IL-17 and Treg related factor TGF- 尾 in serum. The measured data were analyzed by SPSS19.0 software. Results the percentage of Th17 cells in PBMCs in AIH group and PBC group was higher than that in control group (P < 0. 01). The percentage of Th17 cells in PBMCs was significantly lower than that in control group (P < 0. 01). The content of IL-17 in AIH group and PBC group was higher than that in control group (P0. 05), and the content of serum TGF- 尾 was lower than that in control group (P0. 01). Conclusion in AILD patients, the changes of Th17,Treg cells may play an important role in the pathogenesis of AILD, which may be one of the mechanisms that lead to the pro-inflammatory direction of the immune system and lead to the pathogenesis of AILD. The observation of Th17/Treg can be used to monitor the disease progression and observe the curative effect of AILD, IL-17 and TGF- 尾 have strong correlation with AILD, and the combination of IL-17 and TGF- 尾 can improve the sensitivity of AILD diagnosis.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R575
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相关期刊论文 前3条
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3 余海静;黄加权;刘阳;艾国;严伟明;王晓晶;宁琴;;IL-17 Contributes to Autoimmune Hepatitis[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2010年04期
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