银杏总黄酮对NAFLD大鼠脂联素信号通路的影响

发布时间：2019-02-16 18:26

【摘要】：目的探讨银杏总黄酮对非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)大鼠脂联素信号通路的影响。方法利用高脂饲料喂养法构建NAFLD大鼠模型。72只SPF级雄性大鼠分为正常对照组、NAFLD模型组和银杏总黄酮治疗组3组,每组24只。测定各组大鼠体质量及肝指数,光镜观察肝脏组织形态改变,RT-PCR法检测大鼠肝脏AdipoR2mRNA、PPARαmRNA的表达,Western blot法检测各组大鼠肝脏AdipoR2、PPARα、AMPK蛋白表达以及AMPK蛋白磷酸化水平。结果 NAFLD大鼠造模成功。第4周末,与正常对照组大鼠相比,模型组及治疗组大鼠体质量与肝指数均增高(均P0.05),肝脏AdipoR2mRNA、PPARαmRNA的表达水平降低,肝脏AdipoR2、PPARα、AMPK蛋白表达以及AMPK蛋白磷酸化水平降低,差异有统计学意义(均P0.05);模型组与治疗组相比,肝脏AdipoR2mRNA、PPARαmRNA的表达水平差异无统计学意义(均P0.05),肝脏AdipoR2、PPARα、AMPK蛋白表达及AMPK蛋白磷酸化水平差异无统计学意义(均P0.05)。第8、12周末,模型组大鼠体质量与肝指数水平逐渐增高,肝脏AdipoR2mRNA、PPARαmRNA的表达水平,AdipoR2、PPARα、AMPK蛋白表达水平及AMPK蛋白磷酸化逐渐下降;治疗组大鼠体质量及肝指数逐渐接近正常对照组,肝脏AdipoR2mRNA、PPARαmRNA的表达水平增高(均P0.05),AdipoR2、PPARα、AMPK蛋白表达及AMPK蛋白磷酸化水平逐渐增高(均P0.05)。结论银杏总黄酮能控制大鼠体质量与肝指数,提高大鼠肝脏AdipoR2mRNA、PPARαmRNA和AdipoR2、PPARα、AMPK蛋白表达水平,并使AMPK蛋白磷酸化水平升高。推测银杏总黄酮可能通过增强脂联素信号通路的活性,一方面减少肝细胞异常凋亡,另一方面促进线粒体内脂肪酸的氧化,从而减轻NAFLD的肝损伤,阻止NAFLD的进展。
[Abstract]:Objective to investigate the effect of total flavonoids of Ginkgo biloba on adiponectin signaling pathway in (nonalcoholic fatty liver disease,NAFLD rats. Methods 72 SPF male rats were divided into normal control group, NAFLD model group and ginkgo total flavonoid treatment group with 24 rats in each group. The body mass and liver index of each group were measured. The morphologic changes of liver were observed under light microscope. The expression of AdipoR2mRNA,PPAR 伪 mRNA in rat liver was detected by RT-PCR method. The expression of AdipoR2,PPAR 伪, AMPK protein and the phosphorylation of AMPK protein were detected by, Western blot method. Results the model of NAFLD rats was successfully established. At the end of the 4th week, compared with the normal control group, the body mass and liver index of the model group and the treatment group were increased (P0.05), the expression of AdipoR2mRNA,PPAR 伪 mRNA in the liver decreased, and the expression of AdipoR2,PPAR 伪 in the liver decreased. The expression of AMPK protein and the level of phosphorylation of AMPK protein decreased significantly (P0.05). There was no significant difference in the expression of AdipoR2mRNA,PPAR 伪 mRNA between the model group and the treatment group (P0.05), but there was no significant difference in the expression of AdipoR2,PPAR 伪, AMPK protein and the phosphorylation of AMPK protein in the liver (P0.05). At the end of the 812th week, the body mass and liver index of the model group increased gradually, while the expression of AdipoR2mRNA,PPAR 伪 mRNA, AdipoR2,PPAR 伪 and AMPK protein and the phosphorylation of AMPK protein decreased gradually in the liver of the model group. In the treatment group, the body mass and liver index were close to the normal control group, the expression of AdipoR2mRNA,PPAR 伪 mRNA in the liver increased (P0.05), the expression of AdipoR2,PPAR 伪, AMPK protein and the phosphorylation of AMPK protein increased gradually (P0.05). Conclusion Total flavonoids of Ginkgo biloba can control the body mass and liver index of rats, increase the expression of AdipoR2mRNA,PPAR 伪 mRNA, AdipoR2,PPAR 伪 and AMPK protein, and increase the phosphorylation level of AMPK protein. It is speculated that the total flavonoids of Ginkgo biloba may reduce the abnormal apoptosis of hepatocytes by enhancing the activity of adiponectin signaling pathway on the one hand and promote the oxidation of fatty acids in mitochondria on the other hand thereby alleviate the liver injury of NAFLD and prevent the progress of NAFLD.
【作者单位】：武汉市第一医院消化内科;
【基金】：武汉市卫生计生委临床医学科研资助项目(No.2014-92-WX14C05)
【分类号】：R575

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