肝脏靶向表达趋化因子CXCL10抑制ConA诱导的肝脏损伤

发布时间：2019-02-22 08:11

【摘要】：目的:本研究探索趋化因子CXCL10在免疫介导的肝脏损伤方面的作用及其作用机制。方法:尾静脉高压注射CXCL10表达载体72 h后检测肝脏中CXCL10的表达水平;再尾静脉注射刀豆蛋白(Concanavalin A,ConA)诱导免疫介导的肝脏损伤;检测并比较CXCL10表达组和对照组的谷丙转氨酶(Alanine aminotransferase,ALT)水平、组织损伤水平、IFN-γ水平、TNF-α水平及调节性T细胞比例和数量的差异。结果:CXCL10表达载体注射72 h后,肝脏中CXCL10的表达水平显著升高;CXCL10表达质粒预处理可以有效减轻ConA诱导的肝脏损伤,CXCL10表达组小鼠血清中的ALT水平显著低于对照组,CXCL10表达组小鼠血清中IFN-γ和TNF-α的水平明显低于对照组,CXCL10表达组小鼠肝脏中调节性T细胞比例和数量高于对照组。结论:趋化因子CXCL10表达载体预处理可减少炎性细胞因子并减轻ConA诱导的肝脏损伤,对治疗免疫介导的肝炎具有重要意义。
[Abstract]:Aim: to investigate the role and mechanism of chemokine CXCL10 in immune-mediated liver injury. Methods: the expression of CXCL10 in liver was detected 72 hours after injection of CXCL10 expression vector in caudal vein hypertension, and the immune-mediated liver injury was induced by injecting concanavalin (Concanavalin) into caudal vein. The levels of alanine aminotransferase (Alanine aminotransferase,ALT), tissue damage, IFN- 纬, TNF- 伪 and the proportion and quantity of regulatory T cells were detected and compared between CXCL10 expression group and control group. Results: the expression of CXCL10 in liver increased significantly 72 hours after injection of CXCL10 expression vector. Pretreatment of CXCL10 expression plasmid could effectively alleviate the liver injury induced by ConA. The serum ALT level in CXCL10 expression group was significantly lower than that in control group, and the levels of IFN- 纬 and TNF- 伪 in CXCL10 expression group were significantly lower than those in control group. The proportion and quantity of regulatory T cells in liver of CXCL10 expression group were higher than that of control group. Conclusion: pretreatment with chemokine CXCL10 expression vector can reduce inflammatory cytokines and alleviate liver injury induced by ConA, which is of great significance in the treatment of immune-mediated hepatitis.
【作者单位】：安徽医科大学;
【基金】：国家自然科学基金青年基金资助项目(No.81302863)
【分类号】：R575

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