淮安市区退休职工脂肪性肝病患病率调查以及miR-143对HepG2细胞脂代谢的影响

发布时间：2019-04-01 10:45

【摘要】：ALD和NAFLD均存在进展为肝硬化、肝癌、肝功能衰竭的风险,已成为21世纪全球重要的公共健康问题之一。本研究第一部分收集整理了“淮安市区企业和事业单位退休职工”的体检资料,并获取了这一特定人群ALD和NAFLD的发病率 NAFLD确切的发病机制目前尚不清楚。miR-143是最早发现与NAFLD有关的miRNA,有研究表明,miR-143参与机体脂代谢的调节。因此,本研究第二部分探讨了miR-143对HepG2细胞脂代谢的调节作用。第一部分目的：了解淮安市区企事业单位退休职工FLD的患病率及其危险因素。方法：收集2011年9月至2012年12月期间来我院体检中心体检的13112名退体人员的体检资料,内容包括：一般情况、实验室检查、B型超声检查等。结果：1.FLD患病人数5853人,总患病率44.63%,其中男性患病率为41.33%,女性患病率为46.62%,女性患病率显著高于男性,差异有统计学意义；2.FLD组四种高脂血症、IFG、DM、高血压的患病率均显著高于非FLD组；3.多因素Logistic回归分析表明FLD的危险因素包括性别(女)、ALT、IFG、高血糖、TG、LDL-C、高血压。结论：淮安市区企事业单位退休人员FLD的患病率较高,且多与其它代谢类疾病并存,必须加强综合防治。目的：探讨miR-143对HepG2细胞脂代谢的调节作用。方法：体外培养HepG2细胞,培养液中加入FFA,同时转染miR-143及其对照,采用qRT-PCR技术检测HepG2细胞转染miR-143的情况。采用油红O染色观察HepG2细胞内脂质积聚的情况。采用脂蛋白脂肪酶-甘油磷酸氧化酶-过氧化物酶-4-氨基安替比林和酚法(GPO-PAP)法在奥林巴斯(OLYMPUS) AU640全自动生化仪上检测HepG2细胞内TG含量。结果：0.25mmol/L FFA可导致HepG2细胞内TG明显积聚,且呈时间依赖性,miR-143过表达可以显著抑制高FFA状态下HepG2内脂质的积聚。结论：miR-143可能参与了肥胖状态下肝脏细胞脂类代谢紊乱的调节。
[Abstract]:Both ALD and NAFLD have the risk of liver cirrhosis, liver cancer and liver failure, which has become one of the most important public health problems in the 21st century. The first part of this study collected and sorted out the physical examination data of "retired employees of enterprises and institutions in Huai'an City". The exact pathogenesis of the incidence of ALD and NAFLD in this particular population has not been known yet. It has been suggested that miR-143 is involved in the regulation of lipid metabolism in the body, which is the first miRNA, to be found to be associated with NAFLD. Therefore, in the second part of this study, we investigated the effect of miR-143 on lipid metabolism in HepG2 cells. Objective: to investigate the prevalence of FLD and its risk factors among retired employees in Huai'an city. Methods: from September 2011 to December 2012, the physical examination data of 13112 retired persons who came to our hospital from September 2011 to December 2012 were collected, including general condition, laboratory examination, B-mode ultrasound examination and so on. Results: the total prevalence rate of 1.FLD was 44.63%, of which 41.33% was male and 46.62% was female. The prevalence rate in female was significantly higher than that in male (P < 0.05). The prevalence of four kinds of hyperlipidemia and IFG,DM, hypertension in 2.FLD group was significantly higher than that in non-FLD group; Multivariate Logistic regression analysis showed that the risk factors of FLD included sex (female), ALT,IFG, hyperglycemia and TG,LDL-C, hypertension. Conclusion: the prevalence of FLD in retirees of enterprises and institutions in Huaian city is higher and coexists with other metabolic diseases, so it is necessary to strengthen comprehensive prevention and treatment. Aim: to investigate the effect of miR-143 on lipid metabolism in HepG2 cells. Methods: HepG2 cells were cultured in vitro. FFA, was added into the culture medium to transfect miR-143 and its control. QRT-PCR technique was used to detect the transfection of HepG2 cells into miR-143. Lipid accumulation in HepG2 cells was observed by oil red O staining. Lipoprotein lipase-glycerophosphate oxidase-peroxidase-4-amino-antipyrine and phenol (GPO-PAP) method were used to detect the TG content in HepG2 cells on Olympus (OLYMPUS) AU640 automatic biochemical instrument. Results: 0.25mmol/L FFA could induce the accumulation of TG in HepG2 cells in a time-dependent manner. The overexpression of miR-143 could significantly inhibit the accumulation of lipid in HepG2 at high FFA level. Conclusion: miR-143 may be involved in the regulation of lipid metabolism disorder in obese liver cells.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R575.5

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