肉桂多酚对HepG2细胞脂质代谢的影响及其机制

发布时间：2019-05-18 00:05

【摘要】：目的观察肉桂提取物肉桂多酚对HpeG2细胞脂质代谢的影响并探讨其作用机制。方法培养Hep G2细胞,用不同浓度的肉桂多酚处理HpeG2细胞后,分别用油红O染色观察脂质沉积情况及甘油三酯(TG)试剂盒检测TG水平变化;采用q-PCR方法检测肉桂多酚处理HpeG2细胞后脂肪酸从头合成相关基因表达水平;Hep G2细胞转染siRNA沉默SIRT1基因后,用Western blotting方法检测下游通路蛋白腺苷酸活化蛋白激酶(AMPK)、乙酰辅酶A羧化酶(ACC)水平以及从头合成相关基因的表达。结果肉桂多酚可有效降低HpeG2细胞内TG水平,下调脂肪酸从头合成关键转录因子SREBP-1及其下游靶基因FAS、SCD1 mRNA表达(P均0.05);SIRT1基因沉默后,下游通路蛋白AMPK、ACC及其磷酸化水平明显降低,SREBP-1、FAS、SCD1 mRNA表达上调(P均0.05)。结论肉桂多酚可有效改善Hep G2细胞内脂质代谢,该作用可能是通过SIRT1-AMPK-ACC通路抑制脂质从头合成实现的。
[Abstract]:Objective to observe the effect of cinnamon polyphenols on lipid metabolism in HpeG2 cells and to explore its mechanism. Methods Hep G2 cells were cultured and HpeG2 cells were treated with different concentrations of cinnamon polyphenols. The lipid deposition and the level of TG were detected by oil red O staining and TG (TG) kit, respectively. The expression of genes related to fatty acid ab initio synthesis in HpeG2 cells treated with cinnamon polyphenols was detected by q-PCR. After siRNA silencing SIRT1 gene in Hep G2 cells, the level of protein adenylate activated protein kinase (AMPK), acetylcoenzyme A Carboxylase (ACC) and the expression of genes related to ab initio synthesis were detected by Western blotting. Results Cinnamon polyphenols could effectively decrease the level of TG in HpeG2 cells and down-regulate the expression of SREBP-1, a key transcription factor synthesized from fatty acid ab initio, and its downstream target gene FAS,SCD1 mRNA. After silencing of SIRT1 gene, the level of downstream pathway protein AMPK,ACC and its phosphorylation decreased significantly, and the expression of SREBP-1,FAS,SCD1 mRNA was up-regulated (all P 0.05). Conclusion Cinnamon polyphenols can effectively improve lipid metabolism in Hep G2 cells, which may be achieved by inhibiting lipid ab initio synthesis through SIRT1-AMPK-ACC pathway.
【作者单位】：贵州医科大学附属医院;
【分类号】：R575.5

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