杀伤细胞免疫球蛋白样受体基因多态性与原发性胆汁性肝硬化的相关性分析

发布时间：2019-05-22 02:58

【摘要】：目的探讨江苏汉族人群杀伤细胞免疫球蛋白样受体(KIR)基因多态性与原发性胆汁性肝硬化(PBC)的相关性。方法收集2011-06-01/2013-07-31期间江苏省常州三院住院的PBC患者122例和198例相匹配的无血缘关系健康人群外周血标本。采用实时荧光定量PCR检测KIR基因多态性,分析KIR基因及基因型频率在2组人群间的差异。结果 PBC患者KIR2DL2和2DS2基因频率比对照组显著性降低,与降低疾病的患病风险有关。共检测出55种KIR基因型,其中PBC组24种,对照组47种。最常见的基因型为AA1,其次为BX2、BX8、BX4、BX14。对存在于5个或以上个体的11个基因型进行分析,未发现PBC组与对照组之间有显著性差异。结论 KIR2DL2和2DS2的缺失可能是江苏地区汉族人群PBC发病潜在的保护因子。
[Abstract]:Objective to investigate the relationship between killer cell immunoglobulin-like receptor (KIR) gene polymorphism and (PBC) in primary bile cirrhosis in Jiangsu Han population. Methods the peripheral blood samples of 122 PBC patients and 198 unrelated healthy people hospitalized in Changzhou three Hospitals of Jiangsu Province during the period of 2011 / 06 / 01 / 2013 / 07 / 31 were collected. The polymorphism of KIR gene was detected by real-time fluorescence quantitative PCR, and the difference of KIR gene and genotypic frequency between the two groups was analyzed. Results the frequencies of KIR2DL2 and 2DS2 genes in patients with PBC were significantly lower than those in the control group, which was related to the reduction of the risk of disease. A total of 55 KIR genotypes were detected, including 24 in PBC group and 47 in control group. The most common genotype was AA1, followed by BX2,BX8,BX4,BX14.. 11 genotypes in 5 or more individuals were analyzed, and no significant difference was found between PBC group and control group. Conclusion the deletion of KIR2DL2 and 2DS2 may be a potential protective factor for PBC in Jiangsu Han population.
【作者单位】：常州市第三人民医院检验科;江苏大学附属武进人民医院检验科;
【基金】：武进科技计划项目(WS2010012)
【分类号】：R575.22

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