溃疡性结肠炎患者肠道菌群分析和细菌毒素基因检测

发布时间：2019-06-19 19:51

【摘要】：目的:探讨溃疡性结肠炎(UC)患者肠道菌群分布和细菌毒素基因的检出情况。方法:收集UC活动期患者32例、缓解期患者21例和45名健康对照的新鲜粪便标本,采用直接涂片镜检和传统细菌培养鉴定法分析菌群分布,利用PCR检测粪便中6种常见的肠道致病菌毒素基因。结果:直接涂片镜检结果显示,UC活动期组菌群失调率(87.5%)高于对照组(53.3%,χ2=9.957,P=0.003),UC活动期组菌群失调程度高于缓解期组(Z=2.501,P=0.012)。需氧培养结果显示,UC组大肠埃希菌优势生长率(66.0%)低于对照组(88.9%,χ2=7.075,P=0.008),条件致病菌优势生长率(32.1%)明显高于对照组(11.1%,χ2=6.144,P=0.013),变形杆菌属是UC组优势生长率最高的条件致病菌。厌氧培养结果显示,UC组益生菌和条件致病菌优势生长率与对照组比较差异并无统计学意义(P0.05)。UC组6种细菌毒素基因的总检出率(41.5%)明显高于对照组(22.2%,χ2=4.117,P=0.042)。结论:UC患者存在明显的肠道菌群失调。
[Abstract]:Objective: to investigate the distribution of intestinal flora and the detection of bacterial toxin gene in patients with ulcerative colitis (UC). Methods: fresh fecal samples from 32 patients with active UC, 21 patients with remission stage and 45 healthy controls were collected. The distribution of flora was analyzed by direct smear microscopic examination and traditional bacterial culture identification method, and six common intestinal pathogenic bacteria toxin genes in feces were detected by PCR. Results: the results of direct smear examination showed that the rate of flora disorder in UC active group (87.5%) was higher than that in control group (53.3%, 蠂 2 鈮，

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