APETx2对应激性胃黏膜损伤大鼠脊髓背根神经节酸敏感离子通道3表达的影响

发布时间：2019-07-30 14:42

【摘要】：目的:观察酸敏感离子通道3(ASIC3)特异性拮抗剂APETx2对应激性胃黏膜损伤大鼠脊髓背根神经节(DRG)神经元上ASIC3表达的影响。方法:24只雄性Wistar大鼠随机等分为对照组(NC组)、应激模型组(WIRS组)及APETx2处理组(AT组,腹腔内注射APETx2 25μg/kg)。采用浸水束缚应激(WIRS)法复制应激性胃黏膜损伤模型,于应激6 h后留取标本测定胃液p H值;HE染色观察胃组织病理学改变;免疫组化和实时定量PCR法测定DRG神经元上ASIC3蛋白及m RNA表达。结果:与NC组比较,WIRS组胃p H值明显降低(P0.05),胃黏膜损伤程度严重,DRG神经元上ASIC3表达明显上调(P0.05);与WIRS组比较,AT组胃液p H值明显升高(P0.05),胃黏膜损伤程度减轻,DRG神经元上ASIC3表达明显减少(P0.05)。结论 :浸水束缚应激所致胃黏膜损伤大鼠DRG神经元上ASIC3被激活,与胃液p H下降、胃黏膜损伤严重程度密切相关,APETx2可特异性拮抗ASIC3表达,对应激性胃黏膜损伤可能起保护作用。
[Abstract]:Objective: To observe the effect of the specific antagonist of the acid-sensitive ion channel 3 (ASIC3) on the expression of ASIC3 on the dorsal root ganglion (DRG) of the spinal cord of rats with stress-induced gastric mucosal injury. Methods:24 male Wistar rats were randomly divided into control group (NC group), stress model group (WIRS group) and APET2 treatment group (AT group, intra-abdominal injection APETx2 25. mu.g/ kg). The stress-induced gastric mucosal injury model was reproduced by the WIRS method, and the pH of the gastric juice was measured after 6 h of stress. The pathological changes of the gastric tissues were observed by HE staining. The expression of ASIC3 and m-RNA on the DRG neurons was determined by immunohistochemistry and real-time quantitative PCR. Results: Compared with the NC group, the gastric p H value of the WIRS group was significantly lower (P0.05), the degree of gastric mucosal injury was severe, and the expression of ASIC3 on the DRG neurons was significantly increased (P0.05); the gastric juice p H value in the AT group was significantly higher than that of the WIRS group (P0.05), and the degree of gastric mucosal injury was reduced. There was a significant decrease in the expression of ASIC3 on DRG neurons (P0.05). Conclusion: The activation of ASIC3 on the DRG neurons of the rats with gastric mucosal injury caused by water-water-binding stress is closely related to the decrease of the gastric juice p H and the severity of gastric mucosal injury, and the APET2 can specifically antagonize the expression of the ASIC3, and may play a protective role in the stress-induced gastric mucosal injury.
【作者单位】：广州中医药大学第二附属医院麻醉科;广州军区广州总医院麻醉科、全军临床麻醉中心;
【基金】：国家自然科学基金项目(编号:81272141) 广东省自然科学基金项目(编号:S2011010003373)
【分类号】：R573

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