CBP治疗通过调节ROCK介导的细胞间紧密连接蛋白改善SAP患者内皮细胞通透性
发布时间：2021-11-06 00:03
背景：重症急性胰腺炎（SAP）作为一种常见的急腹症，病因复杂，病情进展快，可迅速发展为全身炎症综合症（SIRS），进而发展为多器官功能障碍综合征（MODS），而内皮细胞作为血管的最内层屏障，其细胞间通透性增加可能参与了MODS的发生。细胞间紧密连接蛋白是细胞间连接的重要组成部分，在维持内皮屏障完整中发挥着重要的调节作用。近年来连续性血液净化（CBP）治疗已广泛应用临床抢救包括SAP、MODS在内的各种危重患者的治疗，明显的改善了患者的预后。我们既往的研究显示CBP治疗可以改善SAP患者内皮细胞通透性，但其机制是否与细胞间紧密连接异常表达与分布有关，有待进一步深入研究。本文旨在探讨细胞间紧密连接蛋白在CBP改善SAP患者内皮细胞屏障功能障碍中的作用。方法：用SAP患者CBP治疗的不同时间点血清刺激人脐静脉内皮细胞，或予不同浓度Rho激酶（ROCK）抑制剂Y-27632（5μ M、10μ M、20μ M）预处理后加入SAP治疗前血清干预内皮细胞。Fluorescence reader检测单层内皮细胞对荧光素标记的牛血清白蛋白的通透量，免疫荧光染色检测紧密连接蛋白Occludin、Claud...
【文章来源】：重庆医科大学重庆市
【文章页数】：48 页
【学位级别】：硕士
【部分图文】：
CBP治疗及Y-27632对SAP患者内皮细胞细胞通透性的影响
图 5 CBP治疗及 Y-27632对 SAP患者血清诱导内皮细胞紧密连1.对照组 2.CBP 治疗前组 3.CBP 治疗 6h 组 4. Y-27632(5μM6.Y-27632(20μM)组。#P＜0.05 VS 对照组，*P＜0.05VS CBP 治疗μM)组，★P＜0.05VS Y-27632(10μM)组Figure 5. The effects of CBP and Y-27632 on the expression of tighin endothelial cells induced by serum from SAP patients .Lan1：hPre-CBP group, Lan3：6-hour-CBP group, Lan4：Y-27632（5μM）ggroup, Lan6：Y-27632（20μM）group). Data were presented asgroup，*P＜0.05 vs Pre-CBP group，▲P＜0.05 vsY-27632(5μY-27632(10μM) group.2.4.2 CBP 治疗及 Y-27632 对 SAP 患者内皮细胞 Claudin与对照组相比，SAP 患者治疗前血清刺激内皮细胞后
图 6 CBP治疗及 Y-27632 对 SAP 患者血清诱导内皮细胞紧密连响。注：1.对照组 2.CBP 治疗前组 3.CBP 治疗 6h 组 4. Y-27632组 6.Y-27632(20μM)组。#P＜0.05 VS 对照组，*P＜0.05VS CBY-27632(5μM)组，△P＜0.05VS Y-27632(10μFigure 6. The effects of CBP and Y-27632 on the expression of tighin endothelial cells induced by serum from SAP patients .Lan1：hPre-CBP group, Lan3：6-hour-CBP group, Lan4：Y-27632（5μM）ggroup, Lan6：Y-27632（20μM）group). Data were presented agroup,*P＜0.05 vs Pre-CBP group,★P＜0.05 vs Y-27632(5μY-27632(10μM) group.3 讨论众所周知，SAP 不仅仅是胰腺和胰周组织的局部炎症身炎症反应综合征而并发严重的并发症，如低血压、血流
【参考文献】：
期刊论文
[1]Microcirculation disturbance affects rats with acute severe pancreatitis following lung injury[J]. Xue-Min Liu, Qing-Guang Liu, Jun Xu, Cheng-En Pan, Department of Hepatobiliary Surgery, Xi’an Jiaotong University First Hospital, Xi’an 710061, Shaanxi Province, China. World Journal of Gastroenterology. 2005(39)
[2]Influence of continuous veno-venous hemofiltration on the course of acute pancreatitis[J]. Hong-Li Jiang, Wu-Jun Xue, Da-Qing Li, Ai-Ping Yin, Xia Xin, Chun-Mei Li, Ju-Lin Gao, Department of Hemodialysis Center, The First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. World Journal of Gastroenterology. 2005(31)
[3]Clinical effects of continuous high volume hemofiltration on severe acute pancreatitis complicated with multiple organ dysfunction syndrome[J]. Hao Wang Wei Zhou Wei-Qin Li Ning Li Jie-Shou Li Department of Surgery,School of Medicine,Nanjing University,Nanjing 210002,Jiangsu Province,China. World Journal of Gastroenterology. 2003(09)
本文编号：3478767
【文章来源】：重庆医科大学重庆市
【文章页数】：48 页
【学位级别】：硕士
【部分图文】：
CBP治疗及Y-27632对SAP患者内皮细胞细胞通透性的影响
图 5 CBP治疗及 Y-27632对 SAP患者血清诱导内皮细胞紧密连1.对照组 2.CBP 治疗前组 3.CBP 治疗 6h 组 4. Y-27632(5μM6.Y-27632(20μM)组。#P＜0.05 VS 对照组，*P＜0.05VS CBP 治疗μM)组，★P＜0.05VS Y-27632(10μM)组Figure 5. The effects of CBP and Y-27632 on the expression of tighin endothelial cells induced by serum from SAP patients .Lan1：hPre-CBP group, Lan3：6-hour-CBP group, Lan4：Y-27632（5μM）ggroup, Lan6：Y-27632（20μM）group). Data were presented asgroup，*P＜0.05 vs Pre-CBP group，▲P＜0.05 vsY-27632(5μY-27632(10μM) group.2.4.2 CBP 治疗及 Y-27632 对 SAP 患者内皮细胞 Claudin与对照组相比，SAP 患者治疗前血清刺激内皮细胞后
图 6 CBP治疗及 Y-27632 对 SAP 患者血清诱导内皮细胞紧密连响。注：1.对照组 2.CBP 治疗前组 3.CBP 治疗 6h 组 4. Y-27632组 6.Y-27632(20μM)组。#P＜0.05 VS 对照组，*P＜0.05VS CBY-27632(5μM)组，△P＜0.05VS Y-27632(10μFigure 6. The effects of CBP and Y-27632 on the expression of tighin endothelial cells induced by serum from SAP patients .Lan1：hPre-CBP group, Lan3：6-hour-CBP group, Lan4：Y-27632（5μM）ggroup, Lan6：Y-27632（20μM）group). Data were presented agroup,*P＜0.05 vs Pre-CBP group,★P＜0.05 vs Y-27632(5μY-27632(10μM) group.3 讨论众所周知，SAP 不仅仅是胰腺和胰周组织的局部炎症身炎症反应综合征而并发严重的并发症，如低血压、血流
【参考文献】：
期刊论文
[1]Microcirculation disturbance affects rats with acute severe pancreatitis following lung injury[J]. Xue-Min Liu, Qing-Guang Liu, Jun Xu, Cheng-En Pan, Department of Hepatobiliary Surgery, Xi’an Jiaotong University First Hospital, Xi’an 710061, Shaanxi Province, China. World Journal of Gastroenterology. 2005(39)
[2]Influence of continuous veno-venous hemofiltration on the course of acute pancreatitis[J]. Hong-Li Jiang, Wu-Jun Xue, Da-Qing Li, Ai-Ping Yin, Xia Xin, Chun-Mei Li, Ju-Lin Gao, Department of Hemodialysis Center, The First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. World Journal of Gastroenterology. 2005(31)
[3]Clinical effects of continuous high volume hemofiltration on severe acute pancreatitis complicated with multiple organ dysfunction syndrome[J]. Hao Wang Wei Zhou Wei-Qin Li Ning Li Jie-Shou Li Department of Surgery,School of Medicine,Nanjing University,Nanjing 210002,Jiangsu Province,China. World Journal of Gastroenterology. 2003(09)
本文编号：3478767
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