conjunctival defect xenogeneic acellular dermal matrix heter

  本文关键词：异种脱细胞真皮基质修复结膜缺损的实验研究，由笔耕文化传播整理发布。

异种脱细胞真皮基质修复结膜缺损的实验研究

study on conjunctival defect xenogeneic acellttlar dermal

[1] [2] [3] [4] [5]

GAO Chang-Hua, ZHANG Xiang-Rong, ZHOU Qiong, PEI Chong-Gang, ZHOU Qin

[1]宁德市医院眼科,福建省宁德市352100; [2]南昌大学第一附属医院眼科,江西省南昌市330006

文章摘要：目的观察异种（鼠）脱细胞真皮基质作为结膜生长支架移植材料修复兔结膜缺损的效果，探讨异种脱细胞真皮基质应用于结膜缺损修复和结膜囊狭窄成形术的可行性。方法60只健康新西兰大白兔随机分为实验组（n=30）和对照组（n=30）。实验组和对照组分别移植异种（鼠）脱细胞真皮基质植片和同种异体巩膜植片。于术后1周、2周、4周、8周、12周采集实验兔耳动脉血，离心后行酶联免疫吸附法检测血清中白细胞介素-2（interleukin2，IL-2）、白细胞介素-10（interleukin10，IL-10）含量，同时取移植区组织行HE染色，光镜下观察植片组织学变化。结果实验组和对照组植片均存活，实验组和对照组结膜缺损修复时间分别为（16．33±0．77）d、（15．78±0．88）d，两组比较差异无统计学意义（P=0．056）。组织学检查显示两组术后早期植片中细胞浸润，术后2周异种脱细胞真皮基质中新生血管长入，，术后12周异种脱细胞真皮基质被重建，异体巩膜被吸收。术后实验组与对照组外周血清IL-2、IL-10浓度差异均无统计学意义（均为P〉0．05）；实验组外周血清IL-2、IL-10浓度差异也均无统计学意义（均为P〉0．05）。结论异种脱细胞真皮基质可能成为一种理想的结膜缺损修复和结膜囊狭窄成形术移植材料。

Abstr：Objective To observe the effects of applying xenogeneic （rat） acel- lular dermal matrix（rat-ADM） for conjunctival defect repair of rabbit,and investigate the feasibility of xenogeneic aceUular dermal matrix（xeno-ADM） for the conjunctival defect repair or conjunctival sac reconstruction. Methods Sixty health New Zealand white rabbits were randomly divided into the experimental group （30 cases） and control group （30 cases）. The rabbits in the experimental group and control group were transplanted with rat-ADM graft and the allograft scleral graft, respectively. The peripheral blood were collected in experimental and control groups at 1 week, 2 weeks, 4 weeks, 8 weeks, 12 weeks after the operation, IL-2 and IL-10 concentrations in serum were meas- ured after centrifugation. Meanwhile, the tissues of transplant district were harvested for HE staining,then histologic changes were observed under fight-microscopy. Results The graft in experimental group and control group were all surviving, the conjunctival defect repair time in experimental group and control group were （ 15.33 ± 0.77 ） days and （ 15.78 ± 0.88 ） days, respectively, there was no statistical difference （P = 0.055 ）. His- tological examination showed cells infiltrated in postoperative grafts of two groups ear- ly,new blood vessels grew in ADM grafts at 2 weeks after operation, and ADM grafts were rebuilted and sclera grafts were absorbed gradually at 12 weeks after operation. There was no statistical difference in IL-2 and IL-10 concentrations between two groups （all P 〉 0.05 ）, and no statistical difference between IL-2 and IL-10 concentration in ex- perimental group （ all P 〉 0.05 ）. Conclusion The xeno-ADM could be used as a kind of ideal transpl

文章关键词：

Keyword：:conjunctival defect xenogeneic acellular dermal matrix heterograft

课题项目：基金项目：国家自然科学基金（编号：81160118）;江西省卫生厅科技计划项目（编号：20121043）

  本文关键词：异种脱细胞真皮基质修复结膜缺损的实验研究，由笔耕文化传播整理发布。