miR-28-3p通过PI3K/AKT通路对大鼠糖尿病性视网膜病变及视网膜血管生成的影响（英文）

发布时间：2022-08-01 17:13

　　目的:探讨miR-28-3p通过PI3K/AKT通路对糖尿病性视网膜病变（DR）大鼠及视网膜血管生成的影响。方法:SD大鼠分为假手术组（sham组）、糖尿病模型组（model组）、糖尿病+阴性对照组（miR-NC组）和糖尿病+miR-28-3p inhibitor组（miR-28-3p inhibitor组）,采用腹腔注射链脲佐菌素溶液建立糖尿病大鼠模型并给与腺相关病毒（AAV-U6-inhibitor-rno-miR-28-3pCAG-EGFP）治疗;qPCR检测各组大鼠视网膜组织中miR-28-3p的表达情况;HE染色观察各组大鼠视网膜内血管分布面积情况;免疫荧光检测CD31的表达;免疫组化检测各组大鼠视网膜组织中Bax和Bcl-2蛋白的表达情况;ELISA检测各组大鼠血清中炎性因子（TNF-α、IL-1β和IL-6）的表达情况;Western blotting检测PI3K、AKT蛋白表达情况。结果:与sham组相比,model组大鼠视网膜组织中miR-28-3p的表达上调（P<0.001）,与model组相比,miR-28-3p inhibitor组大鼠视网膜组织中miR-... 

【文章页数】：9 页

【文章目录】：
Introduction
Materials and methods
    Materials
    Methods
        Grouping and administration
        Modeling
            The role of miR-28-3p in angiogenesis in DR rats by HE staining
            Expression of CD31 by immunofluorescence staining
            Detection of Bax and Bcl-2 expression in retina of rats in each group byimmunohistochemistry
            Detection of inflammatory cytokines by ELISA
            Detection of miR-28-3p expression in retina of rats in each group by qPCR
            Detection of PI3K/AKT protein expression in rat retina by Western blotting
        Statistical method
Results
    Expression of miR-28-3p in retina of rats in each group
    Effect of miR-28-3p on intraretinal angiogenesis in DR rats
    Expression of Bax and Bcl-2 proteins in retina of rats in each group
    Expression of inflammatory cytokines in serum of rats in each group
    Expression of PI3K/AKT protein in retina of rats in each group
Discussion


【参考文献】：
期刊论文
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[2]MicroRNAs as biomarkers of diabetic retinopathy and disease progression[J]. Bridget Martinez,Philip V.Peplow.  Neural Regeneration Research. 2019(11)
[3]PI3K/Akt信号转导通路与增殖期糖尿病视网膜病变的关联性及“补肾活血法”的调节作用研究进展[J]. 杨敏,罗向霞,康莉,王晗.  中华中医药学刊. 2018(06)



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