几种果皮提取物的体外抗氧化与抗肿瘤活性比较

发布时间：2018-05-20 15:52

本文选题：水果果皮 + 总酚　；参考：《食品科技》2017年01期

【摘要】：以6种水果果皮为原料,采用热水浸提和AB-8大孔吸附树脂纯化,得到果皮提取物;采用DPPH自由基清除能力和还原能力分别评价果皮提取物体外抗氧化性能;利用MTT法考察果皮提取物对人肝癌Hep G2细胞生长增殖的影响。结果表明,6种果皮提取物的总酚和总黄酮含量范围分别为(22~1696)mg GAE/100 g干重和(21~2047)mg RE/100 g干重,芒果皮的总酚含量和葡萄皮的总黄酮含量最高,分别为含量最低菠萝皮的77.09倍和97.48倍。葡萄皮提取物和芒果皮提取物分别具有最强的DPPH自由基清除能力和还原能力。6种果皮提取物抑制Hep G2细胞增殖的IC50值范围为(29.97~99.13)μg/m L,菠萝皮的抑制活性最弱,其IC50值为抑制活性最强葡萄皮的3.31倍。果皮提取物的总酚和总黄酮含量与其DPPH清除能力(R2=0.3894,R2=0.3721;p0.05)、还原能力(R2=0.5580,R2=0.2237;p0.05)和抑制Hep G2细胞增殖的IC50值(R2=0.9006,R2=0.7413;p0.05)之间具有一定的正相关性。综合分析比较6种果皮提取物可知,葡萄皮的抗氧化与抗肿瘤活性优于其他果皮,表明其可作为一种良好的天然抗氧化剂及抗肿瘤活性物质来源。
[Abstract]:The pericarp extract was obtained from 6 kinds of fruit peel by hot water extraction and AB-8 macroporous adsorption resin, and the antioxidant activity of pericarp extract was evaluated by DPPH free radical scavenging ability and reducing ability, respectively. The effects of pericarp extract on the growth and proliferation of human hepatoma Hep G2 cells were studied by MTT assay. The results showed that the contents of total phenol and total flavonoids in the six kinds of peel extracts were 22 ~ 22 ~ 1696 mg GAE/100 g dry weight and 21 ~ 21 2047 mg RE/100 g dry weight respectively. The total phenol content of mango peel and the total flavonoids content of grape peel were the highest, which were 77.09 times and 97.48 times of the lowest pineapple peel, respectively. Grape peel extract and mango peel extract had the strongest DPPH free radical scavenging ability and reduction ability respectively. The IC50 value range of inhibiting proliferation of Hep G2 cells was 99.13 渭 g / mL, and the inhibitory activity of pineapple peel was the weakest. Its IC50 value was 3.31 times of that of the most active grape skin. There was a positive correlation between the total phenol and total flavonoids content of peel extract and the DPPH scavenging ability of R2O 0.3894, R2N 0.3721 (p0.05), the reduction ability of R2N 0.5580, R2O 0.2237 (p0.05), and the IC50 value of R20.9006R20.7413p0.05) in inhibiting the proliferation of Hep G2 cells. Comprehensive analysis and comparison of 6 peel extracts showed that the antioxidation and antitumor activities of grape peel were superior to those of other pericarp, which indicated that grape peel could be used as a good source of natural antioxidants and antitumor active substances.
【作者单位】：江苏大学食品与生物工程学院;
【基金】：国家自然科学基金项目(31201456,31371734) 江苏省“六大人才高峰”项目(SWYY-022) 江苏省“青蓝工程”项目(1191360002) 江苏省重点研发计划项目(BE2016350) 江苏高校优势学科建设工程项目
【分类号】：R151

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