钙对母鼠氟染毒后子代大鼠肾脏细胞线粒体损伤的影响

发布时间：2018-05-20 21:58

本文选题：钙 + 氟中毒　；参考：《环境与职业医学》2017年02期

【摘要】：[目的]研究饲料钙对母鼠饮水型氟染毒后子代大鼠肾细胞线粒体损伤的影响。[方法]选用健康初断乳SD雌性大鼠100只,随机分为对照组、染氟组(100 mg/L Na F)、低钙组(0.063%CaCO_3)、低钙染氟组(100 mg/L Na F+0.063%CaCO_3)和高钙染氟组(100 mg/L Na F+7%CaCO_3);饲养3个月后,雌雄鼠合笼交配。取日龄14 d及28 d仔鼠雌雄各10只,以其肾脏细胞线粒体标志酶琥珀酸脱氢酶(SDHase)活性及脂质过氧化指标丙二醛(MDA)水平,肾脏细胞凋亡状况,线粒体分裂/融合蛋白Fis1、Drp1和Mfn2表达水平为观察指标。[结果]与染氟组相比,高钙染氟组SDHase活性升高(P0.05),低钙染氟组SDHase活性降低(P0.05)。与对照组相比,雌鼠各组肾脏线粒体MDA含量均升高(P0.05)。与对照组相比,染氟各组仔鼠凋亡细胞增多;与染氟组相比,低钙染氟组凋亡细胞增多,而高钙染氟组凋亡细胞减少。与对照组相比,低钙染氟组14 d雄鼠的分裂蛋白Fis1表达升高(P0.05);低钙染氟组和高钙染氟组28 d雄鼠的分裂蛋白Drp1升高(P0.05)。[结论]氟中毒能够造成大鼠肾脏细胞线粒体内分裂/融合蛋白Fis1、Drp1和Mfn2表达异常,引起肾脏细胞线粒体损伤。高钙饲料摄入能降低线粒体内脂质过氧化反应,减轻高氟对子代肾脏细胞的毒性作用,而低钙饲料摄入会加剧高氟的毒性作用。
[Abstract]:[objective] to study the effect of dietary calcium on mitochondria damage of rat renal cells after drinking fluoride exposure. [methods] 100 healthy female SD rats were randomly divided into three groups: control group (n = 100), 0.063CO_3 group (n = 100) and high Ca ~ (2 +) -exposed group (n = 100 mg/L Na F 7CO_3). Females and males mate in cages. The activity of SDHase and the level of malondialdehyde (MDAs), a marker of succinate dehydrogenase (SDHase) and lipid peroxidation, were measured in 10 male and 10 male rats of 14 d and 28 d, respectively, and the apoptosis of renal cells was observed. The expression levels of Drp1 and Mfn2 in mitochondrial division / fusion protein Fis1 were observed. [results] compared with the fluoride group, the SDHase activity of the high calcium fluoride group was higher than that of the low calcium fluoride group, while the SDHase activity of the low calcium fluoride group was lower than that of the low calcium fluoride group. Compared with the control group, the content of MDA in kidney mitochondria of female rats was higher than that of control group. Compared with the control group, the number of apoptotic cells in fluoride exposed group was higher than that in fluoride group, but the number of apoptotic cells in low calcium fluoride group was higher than that in high calcium fluoride group. Compared with the control group, the expression of mitogen Fis1 in the low calcium fluoride group was higher than that in the control group on the 14th day, and that in the low calcium fluoride group and the high calcium fluoride group at 28 days was higher than that in the control group. [conclusion] fluoride poisoning can cause abnormal expression of Dr p1 and Mfn2 in mitochondria of rat kidney cells, and cause mitochondrial damage of renal cells. High calcium diet can reduce lipid peroxidation in mitochondria and reduce the toxicity of high fluoride to kidney cells of offspring, but low calcium diet can aggravate the toxic effect of high fluoride.
【作者单位】：浙江师范大学化学与生命科学学院;浙江师范大学体育与健康科学学院;浙江师范大学行知学院;
【基金】：国家自然科学基金(编号:81273015,81573101)
【分类号】：R114

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