水体细菌微生物多波长透射光谱快速准确获取方法研究

发布时间：2018-05-24 20:15

本文选题：多波长透射光谱 + 细菌微生物　；参考：《光谱学与光谱分析》2017年09期

【摘要】：为实现水体细菌微生物快速在线监测,搭建了多波长透射光谱快速测量实验系统,利用该系统分别测量了重铬酸钾标准溶液紫外波段及中性滤光片可见波段的透射光谱,并与紫外-可见分光光度计测得的透射光谱进行对比分析,验证了实验系统测量透射光谱的准确性;以水体中常见的金黄色葡萄球菌作为研究对象,利用搭建的实验系统获取金黄色葡萄球菌溶液在220~900nm波段的前向小角度透射光谱,进一步验证了实验系统测量细菌微生物透射光谱的准确性和快速性。结果表明,由实验系统和紫外-可见分光光度计测得的重铬酸钾标准溶液,与中性滤光片紫外波段及可见波段透射光谱的线性拟合相关系数分别为0.999 7和0.999 5,光密度误差分别在5.00%和4.58%以内,说明两个系统测量光谱的一致性较好,所搭建的实验系统测量标准样品紫外-可见透射光谱准确度较高;对于金黄色葡萄球菌,实验系统测得的透射光谱经过校正后,与紫外-可见分光光度计测得的透射光谱线性拟合的相关系数为0.999 97,两者相比的光密度误差在0.74%以内;系统重复30次细菌光谱信号采集获得平均透射光谱单次测量时间为15s,说明该实验系统相对于紫外-可见分光光度计能够快速准确获取水体细菌微生物多波长透射光谱,在保证测量结果准确的同时缩短了光谱测量时间,为水体细菌微生物快速检测提供技术支持。
[Abstract]:In order to realize the rapid on-line monitoring of bacteria and microbes in water body, a multi-wavelength transmission spectrum fast measurement system was set up. The transmission spectra of ultraviolet band and neutral filter band of potassium dichromate standard solution were measured by the system. Compared with the transmission spectrum measured by the UV-Vis spectrophotometer, the accuracy of the experimental system was verified, and the common staphylococcus aureus in water was used as the research object. The low angle forward transmission spectra of Staphylococcus aureus solution in 220~900nm band were obtained by using the established experimental system, which further verified the accuracy and rapidity of the experimental system in measuring the transmission spectrum of bacteria microbes. The results show that the standard solution of potassium dichromate obtained from the experimental system and UV-Vis spectrophotometer, The linear fitting correlation coefficient with the transmission spectrum in the ultraviolet and visible bands of the neutral filter is 0.999 7 and 0.999 5, respectively, and the optical density error is less than 5.00% and 4.58%, respectively, which indicates that the measurement spectra of the two systems are in good agreement. The experimental system has high accuracy in measuring the UV-Vis transmission spectra of standard samples, and for Staphylococcus aureus, the transmission spectra measured by the experimental system have been corrected. The correlation coefficient of linear fitting of transmission spectrum measured by UV-Vis spectrophotometer is 0.999, and the optical density error is less than 0.74%. The average time of single measurement of transmission spectrum is 15 s, which indicates that the system can quickly and accurately obtain the multiwavelength transmission spectrum of bacteria and microorganism in water compared with the UV-Vis spectrophotometer. At the same time, it can shorten the time of spectrum measurement and provide technical support for rapid detection of bacteria and microorganisms in water body.
【作者单位】：中国科学院安徽光学精密机械研究所中国科学院环境光学与技术重点实验室安徽省环境光学监测技术重点实验室;中国科学技术大学;
【基金】：安徽省自然科学基金项目(1508085JGD02,1608085QF137) 安徽省科技重大专项项目(15CZZ04125) 国家自然科学基金项目(61378041)资助
【分类号】：O657.33;R123.1

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