花色苷与蛋白质作用对抗氧化性的影响

发布时间：2018-05-26 07:42

本文选题：花色苷 + 牛血清白蛋白　；参考：《山东师范大学》2013年硕士论文

【摘要】：本文通过紫外-可见吸收光谱、荧光光谱、红外光谱、圆二色谱以及分子对接技术研究了花色苷与蛋白质之间相互作用的作用机制。通过DPPH、FRAP、ORAC三种抗氧化研究方法，分别对两种花色苷在与牛血清白蛋白和酪蛋白杂蛋白作用后对花色苷自身抗氧化能力的影响进行了探讨。对于研究花色苷在保健食品、功能性饮料等领域的应用以及研究花色苷在体内发挥作用的机理等方面具有重要的意义。本论文的主要部分如下： 1.第一章综述部分，主要介绍了花色苷的结构、分类方式以及生理功能，介绍了蛋白质结构的知识以及血清白蛋白和酪蛋白的相关知识，对目前关于花色苷抗氧化性常用研究方法和实验进展进行了汇总，指出了目前关于花色苷在体内发挥抗氧化作用的机制尚存在争论。并且综述了近年来关于小分子物质与蛋白质作用对小分子自身抗氧化能力的影响的实验进展。 2.第二章用紫外-可见吸收光谱、荧光光谱、红外光谱、圆二色谱以及分子对接技术研究了矢车菊素-3-O-葡萄糖苷与牛血清白蛋白相互作用的作用机制。结果表明矢车菊素-3-O-葡萄糖苷与牛血清白蛋白之间属于静态猝灭，，矢车菊素-3-O-葡萄糖苷能够与牛血清白蛋白发生结合，并且计算出了它们之间的结合常数和结合位点数。对热力学公式计算结果及分子对接研究结果进行分析，推断氢键是矢车菊素-3-O-葡萄糖苷与牛血清白蛋白结合的主要作用力类型。同时矢车菊素-3-O-葡萄糖苷与牛血清白蛋白的分子对接模型显示花色苷B环上的羟基参与了结合，根据前人关于花色苷B环羟基是花色苷抗氧化主要作用基团的结论，推测这可能会导致花色苷在与蛋白质作用后无法发挥其良好的抗氧化能力。 3.第三章用DPPH、FRAP、ORAC三种抗氧化能力检测方法，对矢车菊素-3-O-葡萄糖苷以及矢车菊素-3-O-半乳糖苷两种花色苷与牛血清白蛋白相互作用后的抗氧化能力进行了研究。三种抗氧化指标测试结果显示：矢车菊素-3-O-葡萄糖苷与矢车菊素-3-O-半乳糖苷的抗氧化能力差别不显著；在与牛血清白蛋白相互作用后，两种花色苷的抗氧化能力都发生了下降。联系花色苷与血清白蛋白结合机制的研究结果，推测花色苷在体内难以通过自身发挥其良好的抗氧化能力。 4.第四章分别研究了与酪蛋白相互作用对矢车菊素-3-O-葡萄糖苷以及矢车菊素-3-O-半乳糖苷的抗氧化能力的影响。DPPH、FRAP、ORAC等三种抗氧化方法检测的结果都显示在与酪蛋白相互作用后会减弱两种花色苷的抗氧化能力，推测酪蛋白也可能通过某种方式与花色苷发生了结合，并且这种结合可能会对花色苷起到一定的保护作用，使花色苷的功能基团在加工、储存和运输过程中免遭破坏。
[Abstract]:In this paper, the interaction mechanism of anthocyanin and protein was studied by ultraviolet visible absorption spectrum, fluorescence spectrum, infrared spectrum, circular two chromatography and molecular docking technique. Through the three antioxidative methods of DPPH, FRAP and ORAC, two anthocyanins were treated with bovine serum albumin and casein clutter, respectively. The effect of glycosides on their own antioxidant capacity is discussed. It is important to study the application of anthocyanins in health food, functional drinks and other fields, and to study the mechanism of anthocyanin in the body. The main parts of this paper are as follows:
The first chapter of chapter 1. introduces the structure, classification and physiological functions of anthocyanin, introduces the knowledge of protein structure and the related knowledge of serum albumin and casein, summarizes the current research methods and experimental progress on anthocyanin antioxidant activity, and points out that anthocyanins are present in the body. The mechanism of antioxidative action is still controversial, and the recent progress in the effect of small molecule material and protein on the self antioxidant capacity of small molecules is reviewed.
2. second chapters use ultraviolet visible absorption spectrum, fluorescence spectrum, infrared spectrum, circular two chromatograph and molecular docking technique to study the interaction mechanism between the glucoside of -3-O- and bovine serum albumin. The results show that the static quenching between the glucoside of -3-O- and the white egg white of cattle is static, and it is -3-O- grape Glucoside can combine with bovine serum albumin and calculate the binding constant and the number of binding sites. The calculation results of the thermodynamic formula and the results of molecular docking are analyzed. The hydrogen bond is the main force type of the combination of -3-O- glucoside and bovine serum albumin. At the same time, the Genin -3-O- The molecular docking model of glucoside and bovine serum albumin showed that the hydroxyl group on anthocyanin B rings participated in the binding. According to the conclusion that the hydroxyl group of anthocyanin B is the main antioxidant group of anthocyanin, it is presumed that this may cause the anthocyanin to not play its good antioxidant capacity after the action of protein.
3. third chapters, using DPPH, FRAP, and ORAC, three kinds of antioxidant assay methods, the antioxidant capacity of three kinds of anthocyanins, two anthocyanins and bovine serum albumin, were studied. The test results of three kinds of antioxidant indexes showed that the glucoside -3-O- glucoside and C. The antioxidant capacity of -3-O- galactoside was not significant. After the interaction with bovine serum albumin, the antioxidant capacity of two anthocyanins decreased. The results of the association of anthocyanins and serum albumin suggested that anthocyanins are difficult to exert their good antioxidant capacity in the body.
In the 4. fourth chapter, the effects of the interaction of casein on the antioxidant capacity of -3-O- glucoside and -3-O- galactoside were investigated respectively. The results of three antioxidant methods, such as.DPPH, FRAP and ORAC, all showed that after interaction with casein, the antioxidant capacity of two anthocyanins was weakened, and casein was also speculated. It may be associated with anthocyanins in some way, and this combination may protect anthocyanins to a certain extent, so that the functional groups of anthocyanins are not destroyed during processing, storage and transportation.
【学位授予单位】：山东师范大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R151.3

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