石家庄市灌溉用污水对大鼠肝功能的影响

发布时间：2018-05-28 02:17

本文选题：灌溉用污水 + 肝脏　；参考：《环境与健康杂志》2017年07期

【摘要】：目的研究石家庄市灌溉用污水对大鼠肝功能的影响,以期为污水灌溉的安全性评价提供参考。方法采用气相色谱-质谱联用仪和电感耦合等离子体质谱仪测定石家庄市农田污灌水样中的重金属含量和有机污染物成分。将80只SD大鼠随机分成4组,每组20只,雌雄各半,其中3个染毒组分别为25%,50%及100%污水,采用自由饮水方式进行染毒,连续28 d;并以蒸馏水为阴性对照组。分别于染毒前及染毒第7、28天采集大鼠血样,通过全自动生化分析仪测定血清中天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活力。结果水样中铬和铅含量分别超过国标0.78、0.43倍,共检出30种有机化学物,主要为多环芳烃类化合物、邻苯二甲酸酯类、杂环类化合物及苯胺类化合物等。与对照组相比,饮用灌溉用污水28 d时,50%和100%剂量组雄、雌性大鼠血清ALT活力升高,差异有统计学意义(P0.05)。结论石家庄灌溉用污水可引起大鼠肝损伤。
[Abstract]:Objective to study the effect of irrigation wastewater on liver function of rats in Shijiazhuang City, and to provide reference for the safety evaluation of sewage irrigation. Methods the contents of heavy metals and organic pollutants were determined by gas chromatography-mass spectrometry (GC-MS) and inductively coupled plasma mass spectrometer (ICP-MS). 80 Sprague-Dawley rats were randomly divided into 4 groups, 20 rats in each group, half male and half female. Among them, 25 50% and 100% sewage were treated with free drinking water for 28 days, and distilled water was used as negative control group. Blood samples of rats were collected before and 728 days after exposure. The activities of aspartate aminotransferase (AST) and alanine aminotransferase (alt) in serum were measured by automatic biochemical analyzer. Results the contents of chromium and lead in the water samples were 0.78 ~ 0.43 times higher than that of the national standard, and 30 organic chemicals were detected, such as polycyclic aromatic hydrocarbons, phthalates, heterocyclic compounds and aniline compounds. Compared with the control group, the serum ALT activity of male and female rats in 50% and 100% groups were significantly higher than that in the control group at 28 days after drinking water for irrigation, and the difference was statistically significant (P 0.05). Conclusion sewage for irrigation in Shijiazhuang can cause liver injury in rats.
【作者单位】：河北医科大学公共卫生学院劳动卫生与环境卫生教研室;
【基金】：河北省卫生厅医学重点指令项目(20090057)
【分类号】：R12

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