石斑鱼中阿维菌素类药物多残留测定及食用安全风险评估

发布时间：2018-05-28 13:02

本文选题：石斑鱼 + 阿维菌素类药物残留　；参考：《食品科学》2017年22期

【摘要】：建立高效液相色谱-串联质谱同时定量检测石斑鱼血浆、肌肉组织、肝脏组织中阿维菌素、伊维菌素、甲氨基阿维菌素苯甲酸盐方法。样品经乙腈提取,碱性氧化铝固相萃取柱和LC-C18固相萃取柱串联净化,Thermo Hypersil Gold C18色谱柱分离,10 mmol/L乙酸铵-0.1%甲酸溶液和乙腈梯度洗脱,电喷雾正离子模式下以多反应监测方式检测,基质匹配法外标定量。分别以环境水体中阿维菌素上下限质量浓度(4、8 ng/mL)、伊维菌素上下限质量浓度(6、12 ng/mL)作为受试质量浓度开展生物富集、消除实验,并对石斑鱼的食用安全进行了风险评估。结果表明,阿维菌素和伊维菌素在2.5~200 ng/mL范围内,甲氨基阿维菌素苯甲酸盐在0.25~20 ng/mL范围内,线性回归系数均大于0.99。方法检出限分别为2.5、2.5、0.25 ng/mL(血浆),1、1、0.1μg/kg(肌肉组织),2.5、2.5、0.25μg/kg(肝脏组织),方法定量限分别为5、5、0.5 ng/mL(血浆),2、2、0.2μg/kg(肌肉组织),5、5、0.5μg/kg(肝脏组织)。3个添加量的平均回收率为74.6%~93.6%,日内相对标准偏差为2.3%~10.9%,日间相对标准偏差为9.2%~12.6%。阿维菌素、伊维菌素均属于非生物累积性物质,在石斑鱼体内代谢规律相同,均按一级动力学过程从体内消除。本研究条件下,环境水体中药物质量浓度是石斑鱼肌肉组织中药物残留质量浓度及消除时间的重要因素。为保证食用安全,环境水体中阿维菌素质量浓度达到4~8 ng/mL时,石斑鱼浸浴72 h后安全食用时间为22 d;环境水体中伊维菌素质量浓度达到6~12 ng/mL时,石斑鱼浸浴72 h后安全食用时间为39 d。
[Abstract]:A high performance liquid chromatography-tandem mass spectrometry (HPLC / MS) method was developed for the simultaneous determination of abamectin, ivermectin and aminoabamectin benzoate in plasma, muscle and liver of grouper. The samples were extracted by acetonitrile, then purified by LC-C18 column and alkaline alumina solid-phase extraction column. Ten mmol/L ammonium acetate -0.1% formic acid solution and acetonitrile gradient elution were separated on Thermo Hypersil Gold C18 column. The results were detected by multi-reaction monitoring under electrospray positive ion mode. External standard quantification by matrix matching method. The concentration of abamectin at the upper and lower limits of abamectin and the concentration of ivermectin at the upper and lower limits of abamectin and ivermectin were used as bioconcentration and elimination experiments, respectively. The food safety of grouper was evaluated. The results showed that the linear regression coefficients of avermectin and ivermectin in the range of 2.5 ~ 200 ng/mL and methylamino abamectin benzoate in the range of 0.25 ~ 20 ng/mL were all greater than 0.99. Methods the detection limit was 2. 5 / 2. 5 ~ 2. 5ng / mL (plasma) = 0.1 渭 g / kg (muscle tissue = 2. 5 / 2. 5g / kg). (liver tissue). The quantitative limit of the method was 5 ~ 5ng / mL (plasma) = 22. 0. 2 渭 g / mL (muscle tissue = 55 / g / mL). The average recovery of the added dose was 93. 6% in liver tissue, 93. 6% in liver tissue, 2. 310.9% in intraday standard deviation, and 2. 310.9% in intraday time, respectively, and the average recovery was 93. 6 渭 g / kg in liver tissue (liver tissue), and the relative standard deviation was 2. 310.9% in intraday time (RSDs = 2 310.9%). The relative standard deviation was 9. 2% and 12. 6%. Avermectin and ivermectin are non-bioaccumulative substances, which have the same metabolic law in grouper and are eliminated from the body according to the first-order kinetic process. In this study, the mass concentration of drugs in environmental water was an important factor of drug residue concentration and elimination time in muscle tissue of grouper. In order to ensure food safety, when the mass concentration of abamectin in environmental water reaches 4 ~ 8 ng/mL, the safe eating time of grouper is 22 days after 72 h immersion, and the concentration of Ivermectin in environmental water reaches 6 ~ 12 ng/mL. The safe eating time of grouper was 39 days after 72 h immersion.
【作者单位】：厦门大学材料学院;福建省水产研究所福建省海洋生物增养殖与高值化利用重点实验室;
【基金】：福建省海洋经济创新发展区域示范项目(闽台重要海洋生物资源高值化开发技术公共服务平台,2014FJPT01) 厦门南方海洋研究中心项目(福建重要海洋经济生物种质库与资源高效开发技术公共服务平台,14PZY017NF17) 福建省海洋与渔业科技项目(KJXH-2010-007) 福建省海洋高新产业发展专项(闽海洋高新[2014]18号) 国家海洋局海洋公益性行业科研专项(201505034-4)
【分类号】：R155.5

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