青岛市某高校大学生叶黄素摄入量及叶黄素壳聚糖脂质体代谢动力学研究

发布时间：2018-05-29 04:32

  本文选题：叶黄素 + 大学生　； 参考：《青岛大学》2017年硕士论文

【摘要】：目的:对青岛市某高校大学生的膳食摄入情况进行调查,了解其膳食叶黄素摄入水平及相关因素。制备叶黄素壳聚糖脂质体,评价其在大鼠体内的生物利用率。方法:1.以青岛市某高校为调查点,随机抽取488名在校大学生进行问卷调查,问卷包括大学生的一般情况、饮食习惯和膳食调查等内容。膳食调查采用3天24小时膳食回顾法,食物重量的估计参照《回顾性膳食调查辅助参照食物图谱》,结合现场实物展示及从餐厅和超市采购的食物所拍摄的图片,对调查对象进行统一培训,并有专业的调查人员现场指导,对调查对象进行统一培训,并有专业的调查人员现场指导。2.采用乙醚注入法制备叶黄素脂质体,制备过程中考察磷脂和胆固醇比、叶黄素和磷脂比、磷脂浓度、p H值、剪切速度等因素对脂质体包封率的影响,经正交试验筛选最佳制备条件。将最佳条件制备的叶黄素脂质体采用壳聚糖溶液进行修饰,制备叶黄素壳聚糖脂质体。3.两月龄健康雄性SD大鼠28只,按照体质量随机分为2组,每组12只(4只留作备用及空白取血),分别采用叶黄素油溶液和叶黄素壳聚糖脂质体给大鼠灌胃,于1h,2h,4h,6h,8h,12h,16h,24h分别采集大鼠血,采用高效液相色谱法(HPLC)测定大鼠血浆中的叶黄素浓度,比较叶黄素的两种剂型在大鼠体内的吸收率。结果:1.在校大学生膳食叶黄素摄入量的中位数为2.14mg/d。膳食叶黄素主要由蔬菜提供,其主要来源是为韭菜、菠菜、西兰花、油菜、豆角和鸡蛋等,而大学生平均每天摄入量较多的蔬菜依次为土豆、番茄、豆角、白菜等单位质量叶黄素含量较低的食物。分析大学生膳食叶黄素摄入量相关的因素,结果发现体质指数(BMI)过高及过低、偏食不爱吃蔬菜、睡眠时间超过8h、患有慢性消化系统疾病等会导致大学生叶黄素摄入量偏低;性别、专业、食欲、偏食不爱吃水果、运动时间等对大学生叶黄素摄入量的影响差异无统计学意义(P0.05)。2.叶黄素脂质体的最佳工艺处方为磷脂与胆固醇比为5:1,叶黄素与磷脂比为1:10,磷脂的浓度为4%,磷酸盐缓冲液的p H值为6.5,剪切速度为12000r/min,剪切时间为3min。此配方制备的叶黄素脂质体包封率为97%,粒径为87nm,叶黄素壳聚糖脂质体的包封率为98.26%,粒径为116nm,脂质体近似球形,粒径均一,4℃存放30天未见沉淀析出,外观均匀。3.动物实验结果显示:叶黄素油溶液和叶黄素壳聚糖脂质体在大鼠血浆中的达峰时间分别为3.8h和5.7h,药时曲线下面积(AUC_(0-t))分别为0.86μg/ml·h和1.04μg/ml·h,以叶黄素油溶液为参比制剂,叶黄素采用壳聚糖脂质体包封后,其相对生物利用度达到了120.8%。结论:1.本次调查的大学生膳食叶黄素的摄入量较低,建议加强营养教育,合理膳食,适当摄入叶黄素含量较高的食物。2.叶黄素壳聚糖脂质体提高了叶黄素的水溶性、稳定性及在大鼠体内的生物利用率。
[Abstract]:Objective: to investigate the dietary intake of lutein among college students in Qingdao. Lutein chitosan liposomes were prepared to evaluate their bioavailability in rats. Method 1: 1. A total of 488 college students were randomly selected from a university in Qingdao to investigate the general situation, dietary habits and diet of college students. The dietary survey was conducted with a 3-day 24-hour diet review method. The weight of the food was estimated with reference to the Food Atlas of the retrospective Dietary Survey, and the images taken from the food purchased from restaurants and supermarkets were combined with the physical display on the spot. Conduct unified training to the investigation object, and have the professional investigator on-site instruction, carry on the unified training to the investigation object, and have the professional investigator spot instruction. Lutein liposomes were prepared by ether injection. The effects of phospholipid / cholesterol ratio, lutein / phospholipid ratio, phospholipid concentration / H value and shear rate on the entrapment efficiency of liposomes were investigated. The optimum preparation conditions were selected by orthogonal test. The xanthophyll liposome prepared under the optimum conditions was modified with chitosan solution to prepare xanthophyll chitosan liposome. 3. Twenty-eight healthy male SD rats of two months old were randomly divided into two groups according to their body weight. 12 rats in each group were divided into two groups: 4 rats in each group were reserved for blood collection and 4 rats in each group were treated with lutein oil solution and lutein chitosan liposome respectively. The blood samples were collected from the rats at 1h, 2h, 2h, 4h, 6h, 8h, 8h, 12h, 16h, 24h, respectively. The concentration of lutein in rat plasma was determined by high performance liquid chromatography (HPLC), and the absorptivity of lutein in rats was compared. The result is 1: 1. The median dietary lutein intake of college students was 2.14 mg / d. The main sources of dietary lutein are Chinese chives, spinach, broccoli, rape, beans and eggs, while the average daily intake of vegetables for college students is potatoes, tomatoes, beans, etc. Chinese cabbage and other food with low lutein content per unit mass. The factors related to dietary lutein intake of college students were analyzed. The results showed that high and low body mass index (BMI), partial eating and not eating vegetables, sleeping time more than 8 hours, chronic digestive system diseases, etc., would lead to low intake of lutein in college students. Sex, specialty, appetite, partial eating and not eating fruit, exercise time and so on had no statistical difference on the intake of lutein in college students. The optimum technological formulation of lutein liposome is that the ratio of phospholipid to cholesterol is 5: 1, the ratio of lutein to phospholipid is 1: 10, the concentration of phospholipid is 4, the pH value of phosphate buffer is 6.5, the shear rate is 12000rmin, and the shearing time is 3min. The entrapment efficiency of lutein liposome prepared by this formulation was 97 nm, the particle size was 87 nm, the entrapment efficiency of lutein chitosan liposome was 98.26, the particle size was 116 nm, the liposome was nearly spherical, and the particle size was uniform at 4 鈩，

本文编号：1949502