虱螨脲原药大鼠亚慢性经口毒性试验

发布时间：2018-05-30 08:55

本文选题：虱螨脲原药 + 亚慢性毒性　；参考：《毒理学杂志》2015年06期

【摘要】：目的研究虱螨脲原药主要亚慢性经口毒性作用,取得虱螨脲原药亚慢性经口的最大无作用剂量和最小有作用剂量参数,初步确定毒作用的靶器官。方法采用经口喂饲法染毒,设3个染毒剂量组和1个空白对照组,雌、雄各半,分别用含500、2 000和8 000 mg/kg虱螨脲原药的加药饲料对大鼠连续经口喂饲染毒90 d,观察动物的一般表现、体重、进食量,试验结束时进行血常规、尿常规、血生化指标、脏器重量、脏器系数以及病理组织学检查。结果血常规检查结果显示,中、高剂量组雌鼠白细胞计数(WBC)降低(P0.05和0.01),雄鼠白细胞计数(WBC)、淋巴细胞比例(LYM)降低(P0.01)、中性粒细胞比例(MON)增高(P0.01),高剂量组雄鼠单核细胞(GRA)比例明显增高(P0.05);血液生化检查结果显示,高剂量组雌鼠尿素氮(BUN)、肌酐(CREA)升高(P0.01),中、高剂量组雄鼠总胆固醇(CHO)、尿素氮(BUN)增高(P0.01);脏器系数检查结果显示,中、高剂量组雌鼠肝体比值增高(P0.05和0.01),雄鼠肝体比值增高(P0.01),睾丸及睾丸体重比值降低(P0.05);病理组织学检查结果显示,中、高剂量组雄鼠睾丸曲精小管生精上皮变性、坏死或消失、睾丸萎缩、间质增生,睾丸病变发生率增高(P0.05);高剂量组雄鼠肝脏脂肪变性发生率增加(P0.01);各剂量组尿常规检查结果显示与对照组比较,差异无统计学意义(P0.05)。低剂量组雌、雄鼠各项指标与其对照组相比,均未见明显差异或有临床意义的改变。结论虱螨脲原药对大鼠的血液、肝、肾、生殖器官、神经系统具有毒性作用。其对SD大鼠亚慢性(90 d)经口毒性的最大无作用剂量雌、雄分别为为41.4和37.9 mg/kg、最小有作用剂量雌、雄分别为为152.0和147.8 mg/kg。
[Abstract]:Objective to study the main subchronic oral toxicity of Licia ureas, obtain the maximum non action dose and minimum dose parameter of the subchronic oral acaron drug, initially determine the target organ of the toxic effect. Methods by oral feeding, 3 dose groups and 1 blank control groups were set up, female and male were half with 500,2 00, respectively. 0 and 8000 mg/kg acarin feed was fed to rats for 90 d. The general performance, weight, food intake, blood routine, urine routine, blood biochemical index, organ weight, organ coefficient and histopathology examination were observed at the end of the test. Results the results of blood routine examination showed that in the high dose group, the female mice white were white. Cell count (WBC) decreased (P0.05 and 0.01), white cell count (WBC), lymphocyte proportion (LYM) decreased (P0.01), neutrophils ratio (MON) increased (P0.01), and the proportion of mononuclear cells (GRA) in high dose group increased significantly (P0.05). The results of blood biochemical examination showed that the urea nitrogen (BUN) and creatinine (CREA) increased (P0.01) in the high dose group of female rats (P0.01), in the high dose group. In high dose group, the total cholesterol (CHO) and urea nitrogen (BUN) increased (P0.01). The results of organ coefficient examination showed that the ratio of liver body increased (P0.05 and 0.01) in the high dose group (P0.05 and 0.01), the ratio of liver body in male rats increased (P0.01) and the ratio of testis and testis decreased (P0.05). Histopathological examination showed that the high dose group of male rat testis testis tubule tubule Degeneration, necrosis or disappearance of spermatogenic epithelium, atrophy of testis, interstitial hyperplasia and higher incidence of testicular disease (P0.05); the incidence of fatty degeneration in the liver of male rats increased (P0.01) in high dose group (P0.05). The results of urine routine examination in each dose group showed no significant difference between the control group and the control group. Conclusion there were toxic effects on the blood, liver, kidney, reproductive organs and nervous system of rats. The maximum dose of male and male of the subchronic (90 d) SD rats were 41.4 and 37.9 mg/kg, the smallest dose was 152 and 147., respectively. 8 mg/kg.
【作者单位】：湖南省职业病防治院;
【分类号】：R114

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