纳米二氧化钛对金鱼脑组织的氧化应激损伤

发布时间：2018-05-31 01:36

本文选题：纳米二氧化钛 + 氧化损伤　；参考：《环境科学研究》2015年06期

【摘要】：为探讨光催化型nano-Ti O2(纳米二氧化钛)对金鱼(Carassius auratus L.)脑组织的氧化应激损伤效应,以5 nm的锐钛型钛白粉为试验材料,分别在ρ(nano-Ti O2)为0、16、32、64、128 mg湓L下对金鱼进行处理,测定金鱼脑组织MDA(丙二醛)、PC(羰基化蛋白)和H2O2含量,SOD(超氧化物歧化酶)和GPx(谷胱甘肽过氧化物酶)活性以及Cu湓Zn-SOD蛋白表达量.结果表明:ρ(nano-Ti O2)从0 mg湓L升至128 mg湓L,金鱼脑组织Cu湓Zn-SOD活性从1.70×10-3kat湓kg(以鲜质量计,下同)降至0.35×10-3kat湓kg,再回升至0.46×10-3kat湓kg;Mn-SOD活性呈降—升—降趋势,MDA含量从0.85 nmol湓g升至2.83 nmol湓g,之后降至2.54nmol湓g;PC含量一直上升;H2O2含量从4.32μmol湓g升至9.28μmol湓g,之后降至8.38μmol湓g.随着ρ(nano-Ti O2)的加大,Cu湓ZnSOD蛋白表达量逐渐减少.研究表明,nano-Ti O2对金鱼脑组织具有很强的氧化损伤作用,MDA与PC含量升高与Cu湓Zn-SOD和GPx活性下降相关,H2O2含量升高与GPx活性下降等因素有关.
[Abstract]:In order to investigate the effect of photocatalytic nano-Ti O 2 (nanometer titanium dioxide) on Carassius auratus L. The oxidative stress injury effect of brain tissue was studied. The goldfish were treated with 5nm anatase titanium dioxide at 蟻 _ (nano-TiO _ 2) = 0.16 ~ 32o _ (64) 8 mg / L, respectively. The contents of malondialdehyde (malondialdehyde) PCand H2O2 (superoxide dismutase) and glutathione peroxidase (Glutathione peroxidase) in the brain tissue of goldfish were measured. The expression of Cu Zn-SOD protein was measured. The results showed that 蟻 nano-TiO _ 2 increased from 0 mg / L to 128mg / L, Cu Zn-SOD activity in the brain tissue of goldfish increased from 1.70 脳 10-3kat / kg to 128mg / L, respectively. The content of Mn-SOD decreased from 0.85 nmol / g to 2.83 nmol / g, and then decreased from 4.32 渭 mol / g to 9.28 渭 mol gage, and then decreased to 8.38 渭 mol / g. after that, the content of Mn-SOD increased from 0.35 脳 10-3kat / kg to 0.46 脳 10-3kat / kg / kg, and then increased from 0.85 nmol / g to 2.83 nmol / g, and then to 2.54nmol / GPP / g. from 4.32 渭 / mol / g to 9.28 渭 / mol / g. then to 8.38 渭 / mol / g. With the increase of 蟻 -nano-TiO _ 2), the expression of ZnSOD protein decreased gradually. The results showed that nano-TiO _ 2 had a strong oxidative damage effect on the brain tissue of goldfish. The increase of MDA and PC content was related to the decrease of Cu Zn-SOD and GPx activity and the increase of H _ 2O _ 2 content and the decrease of GPx activity.
【作者单位】：安庆师范学院资源环境系;
【基金】：安徽省教育厅自然科学研究项目(044-K08015000009)
【分类号】：R114

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