顺铂诱发大鼠睾丸毒性的机制及葡萄籽原花青素的防护作用

发布时间：2018-06-02 06:07

本文选题：顺铂 + 葡萄籽原花青素　；参考：《食品科学》2015年15期

【摘要】：目的:探究葡萄籽原花青素(grape seed proanthocyanidin extracts,GSPE)对顺铂(cisdiamminedichloroplatinum,CDDP)诱发大鼠睾丸氧化损伤和细胞凋亡的防护作用。方法:将40只大鼠随机分为空白对照组、CDDP模型组、GSPE低剂量组(200 mg/(kg·d))、GSPE高剂量组(400 mg/(kg·d))。分别以蒸馏水和相应剂量的GSPE对大鼠连续灌胃15 d,灌胃10 d后一次性腹腔注射CDDP,剂量为7 mg/kg,空白对照组注射相同剂量的生理盐水。注射CDDP后第5天处死大鼠,检测精子指标、大鼠睾丸组织中超氧化物歧化酶(superoxide dismutase,SOD)与谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、还原型谷胱甘肽(glutathione,GSH)与丙二醛(malondialdehyde,MDA)含量,流式细胞仪检测大鼠睾丸细胞凋亡率,并观察睾丸组织在光镜下形态的变化。结果:CDDP模型组大鼠睾丸和附睾的质量、附睾精子的浓度和活动度、GSH-Px、SOD活力以及GSH含量均显著降低,而MDA水平和睾丸细胞凋亡率显著升高。预防性口服GSPE显著改善了CDDP引起的大鼠睾丸质量减小、功能减弱以及氧化应激、脂质过氧化和细胞凋亡这些不利影响。结论:GSPE对CDDP诱发的大鼠睾丸毒性有剂量依赖性防护作用,其机制很可能与抑制体内氧化应激及细胞凋亡有密切关系。
[Abstract]:Objective: To explore the protective effect of grape seed proanthocyanidin extracts (GSPE) on the oxidative damage and apoptosis of rat testis induced by cisplatin (cisdiamminedichloroplatinum, CDDP). Methods: 40 rats were randomly divided into blank control group, CDDP model group, GSPE low dose group (200 mg/ (kg d)), and high dose group (400) G/ (kg. D)). The rats were treated with distilled water and the corresponding dose of GSPE for 15 d. After 10 d, CDDP was injected into the abdominal cavity and the dosage was 7 mg/kg. The blank control group was injected with the same dose of physiological saline. The rats were killed fifth days after the injection of CDDP, and the sperm indexes were detected, and the superoxide dismutase (superoxide dismutase, S) in the testis tissues of the rats OD) and glutathione peroxidase (glutathione peroxidase, GSH-Px) activity, reduced glutathione (glutathione, GSH) and malondialdehyde (malondialdehyde, MDA) content, flow cytometry to detect the apoptosis rate of rat testicular cells, and observe the morphological changes of testicular tissue under light microscope. Results: the quality of testis and epididymis in the CDDP model group. The concentration and activity of epididymal sperm, GSH-Px, SOD activity and GSH content were significantly decreased, while the MDA level and the apoptosis rate of testis cells increased significantly. Preventive oral GSPE significantly improved the negative effects of CDDP induced rat testicular mass, dysfunction and oxidative stress, lipid peroxisome and apoptosis. Conclusion: GSPE to CDD P induced testicular toxicity in rats has a dose-dependent protective effect, which may be closely related to inhibition of oxidative stress and apoptosis in vivo.
【作者单位】：北京联合大学生物活性物质与功能食品北京市重点实验室;
【基金】：北京联合大学校级科研项目重点实验室开放课题(Zk70201502)
【分类号】：R151

【共引文献】