蓝莓花色苷对东莨菪碱诱导小鼠学习记忆障碍的影响

发布时间：2018-06-02 12:56

本文选题：蓝莓花色苷 + 东莨菪碱　；参考：《食品研究与开发》2017年02期

【摘要】：研究蓝莓花色苷对东莨菪碱诱导小鼠学习记忆障碍的影响,并初步探讨其作用机制。雄性KM种小鼠60只,随机分为6组,分别为空白对照组、模型对照组、阳性对照组(吡拉西坦片200 mg/kg·d)、蓝莓花色苷高剂量组(400 mg/kg·d)、蓝莓花色苷中剂量组(200 mg/kg·d)、蓝莓花色苷低剂量组(100 mg/kg·d),各组连续给药14 d,从实验第15天开始,除空白对照组外,其余各试验组均注射东莨菪碱2 mg/kg,建立小鼠记忆障碍模型,末次给药2 h后,进行跳台试验训练。测试完毕,断头处死小鼠,检测蓝莓花色苷对脑组织乙酰胆碱酯酶(Ach E)活性的影响,免疫印迹法检测海马组织NF-κB p65和Cleaved caspase-3的影响。结果表明:蓝莓花色苷各剂量组能够明显延长小鼠跳台潜伏期,减少5 min内出现的错误次数(P0.05),且可以降低小鼠脑组织海马及皮层ACh E活性(P0.05)。免疫印迹结果表明,蓝莓花色苷能够降低东莨菪碱诱导小鼠海马组织NF-κB p65和Cleaved caspase-3蛋白的表达。蓝莓花色苷对东莨菪碱模型小鼠的学习记忆能力具有一定的改善作用,其机制可能通过影响NF-k B信号转导通路而抑制东莨菪碱诱导的氧化应激和炎性反应。
[Abstract]:To study the effect of blueberry anthocyanin on scopolamine induced learning and memory impairment in mice and its mechanism. Sixty male km mice were randomly divided into 6 groups: blank control group and model control group. The positive control group (Piracetam 200 mg/kg), blueberry anthocyanin high dose group (400 mg/kg / d), blueberry anthocyanin middle dose group (200 mg/kg / d), blueberry anthocyanin low dose group (100 mg/kg / d) were administered continuously for 14 days, except the blank control group, from the 15th day of the experiment. All the other experimental groups were injected scopolamine 2 mg / kg to establish the model of memory impairment in mice. After the last administration for 2 hours, the jumping test was conducted. The mice were killed by decapitation, and the effects of blueberry anthocyanin on the activity of acetylcholinesterase (Ach E) in brain tissue and the effects of NF- 魏 B p65 and Cleaved caspase-3 in hippocampal tissue were detected by Western blot. The results showed that blueberry anthocyanin could significantly prolong the latency of jumping stage, reduce the number of errors occurred within 5 min and decrease the activity of ACh E in hippocampus and cortex of mice. Western blot showed that blueberry anthocyanins could reduce the expression of NF- 魏 B p65 and Cleaved caspase-3 protein in hippocampus of mice induced by scopolamine. Blueberry anthocyanin can improve the learning and memory ability of scopolamine model mice, and its mechanism may inhibit the oxidative stress and inflammatory response induced by scopolamine by affecting NF-k B signal transduction pathway.
【作者单位】：吉林医药学院药物化学教研室;
【基金】：吉林省教育厅资助项目(20156429) 国家自然科学基金资助项目(21102055)
【分类号】：R151

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