饮食铁含量和有氧运动对雌性大鼠血液及海马铁状态的影响

发布时间：2018-06-03 07:26

本文选题：运动 + 雌性大鼠　；参考：《广州医科大学》2013年硕士论文

【摘要】：研究目的本实验通过给予不同铁含量饮食以及长期低强度游泳运动，研究雌性大鼠血液铁状态及海马NHI含量的变化和意义。研究方法实验动物为两批，第一批为断乳SD雌性大鼠60只，随机数字表法均分为饮食低铁含量组（12mg/kg，SL2）、标准铁含量组（45mg/kg，SS2）、高铁含量组（1000mg/kg，SH2），每组均为静息组。第二批为断乳雌性SD大鼠108只，分为饮食低铁含量组（12mg/kg），饮食标准铁含量组（45mg/kg）和饮食高铁含量组（1000mg/kg）。每组再分为运动组和静息组，即分为六组，分别为低铁含量饮食+运动（EL）、低铁含量饮食+静息（SL）、标准铁含量饮食+运动（ES）、标准铁含量饮食+静息（SS）、高铁含量饮食+运动（EH）和高铁含量饮食+静息（SH），各组用相应的铁含量饲料喂养1个月后，运动组开始游泳，每周5天，每天1次，，持续3个月。静息组除不进行运动外，其余处理同对应运动组。运动期满后，大鼠空腹24h，在戊巴比妥钠麻醉下取静脉血，测定红细胞相关指标，包括血红蛋白（Hb）、红细胞压积（HCT）、平均红细胞体积（MCV）、平均红细胞血红蛋白量（MCH）、红细胞分布宽度（RDW）和血清铁状态指标，包括血清铁（SI）、血浆总铁结合力（TIBC）并计算转铁蛋白饱和度（TS）。取海马组织液氮速冻，-80°C冰箱保存，测定海马NHI。研究结果 1.饮食铁含量和长期运动对雌性大鼠血液铁状态的影响饮食铁含量对Hb、HCT、MCV、MCH、RDW、TIBC、TS（P0.01）和SI（P0.05）均有显著影响，运动对Hb、HCT、MCH、SI和TS均有影响（P0.05）。与SS组比较，SL组MCV及MCH显著降低（P0.01），TS降低（P0.05），RDW及TIBC均显著升高（P0.01）；SH组Hb降低（P0.05），HCT升高（P0.05），MCH、RDW、SI、TIBC和TS均无差异（P0.05）。与ES组比较，EL组MCV、MCH、SI、TS均显著降低（P0.01）， TIBC及RDW均显著升高（P0.01）；EH组Hb升高（P0.05），HCT、MCV、MCH、RDW、SI、TIBC和TS均无差异（P0.05）。与SL组比较，EL组SI、TS显著降低（P0.01），Hb、HCT、MCV、MCH升高（P0.05），RDW无差异（P0.05）。与SS组比较，ES组Hb、HCT、MCV、MCH均升高（P0.05），RDW、SI、TIBC、TS均无差异（P0.05）。与SH组比较，Hb、HCT、MCV、MCH、RDW、SI、TIBC和TS均无差异（P0.05）。 2.饮食铁含量和长期运动对雌性大鼠海马铁状态的影响饮食铁含量对海马NHI有显著影响（P0.01），运动对海马NHI无显著影响（P0.05）。与ES组比较，EL组NHI显著降低（P0.01），EH组无明显变化（P0.05）；与SS组比较，SL组NHI显著降低（P0.01），SH组无明显变化（P0.05）；与相应的静息组比，EL、ES、EH组均无明显变化（P0.05）。与第一批静息组比较，EH组NHI升高（P0.05)，ES、EL组无明显变化（P0.05)；SH组NHI显著升高（P0.01)，SS、SL组无明显变化（P0.05)。研究结论 1.长期低铁含量饮食会导致大鼠营养性铁缺乏。 2.在低铁含量饮食时，长期运动会加剧大鼠血清铁状态的降低；在铁含量饮食足够时，长期运动会导致Hb、HCT升高，但血清铁无变化；在高铁含量饮食时，长期运动对血液铁状态均无影响。 3.运动和饮食铁含量两因素之间对雌性大鼠有交互作用，饮食铁含量对海马NHI有影响，低铁含量饮食组海马NHI含量降低。在低铁含量饮食时，长期运动加剧海马NHI的降低；在饮食铁含量充足及高铁含量饮食时，运动后海马NHI无显著变化。 4.在低铁含量饮食和高铁含量饮食时，运动前海马NHI均明显降低，运动后低铁含量降低，而高铁含量饮食恢复正常水平，提示在高铁含量饮食时，运动不对雌性大鼠海马NHI造成影响，海马具有维持铁代谢的机制。
[Abstract]:research objective
This experiment was conducted to investigate the changes of blood iron status and hippocampal NHI content in female rats by giving different iron content diet and long-term low intensity swimming exercise.
research method
The experimental animals were two batches and the first batch of 60 female SD rats were weaned. The random digital table method was divided into the diet low iron content group (12mg/kg, SL2), the standard iron content group (45mg/kg, SS2), the high iron content group (1000mg/kg, SH2), each group was resting group. The second batch of female SD rats were divided into the diet low iron content group (12mg/kg), the dietary standard iron. The content group (45mg/kg) and the diet high iron content group (1000mg/kg) were divided into exercise group and resting group, which were divided into six groups: low iron content diet + exercise (EL), low iron content diet + resting (SL), standard iron content diet + exercise (ES), standard iron content diet + resting (SS), high iron content diet + exercise (EH) and high iron content diet + resting (SH) after feeding on the corresponding iron content feed for 1 months, the exercise group began to swim, 5 days a week, 1 times a day, and lasted 3 months. The rest group was treated with the corresponding exercise group except for no exercise. After the expiration of the exercise period, the rats had a fasting 24h, and the venous blood was taken under pentobarbital anesthesia, and the red blood cell related indexes, including hemoglobin (Hb), were measured. Red blood cell pressure (HCT), average red cell volume (MCV), average red blood cell hemoglobin (MCH), red cell distribution width (RDW) and serum iron status index, including serum iron (SI), plasma total iron junction force (TIBC) and calculation of transferrin saturation (TS). The liquid nitrogen freezer in the hippocampus, -80 / C refrigerator and NHI. for the determination of NHI. in the hippocampus
Research results
1. effects of iron content and long-term exercise on iron status in female rats
Dietary iron content has significant effects on Hb, HCT, MCV, MCH, RDW, TIBC, TS (P0.01) and SI (P0.05), and exercise has an effect on Hb, Hb, RDW, and TIBC.
Compared with group SS, MCV and MCH in SL group decreased significantly (P0.01), TS decreased (P0.05), RDW and TIBC increased significantly (P0.01), SH group Hb decreased.
Compared with the ES group, the MCV, MCH, SI and TS in the EL group were significantly decreased (P0.01), TIBC and RDW increased significantly (P0.01).
Compared with group SL, SI and TS in group EL were significantly decreased (P0.01), Hb, HCT, MCV and MCH increased (P0.05), and there was no difference in RDW (RDW).
Compared with group SS, ES, Hb, HCT, MCV and MCH all increased (P0.05), RDW, SI, TIBC, and TS were not different (P0.05).
Compared with group SH, there was no difference in Hb, HCT, MCV, MCH, RDW, SI, TIBC and TS (P0.05).
2. effect of dietary iron content and long-term exercise on hippocampal status in female rats
Dietary iron content had a significant effect on hippocampal NHI (P0.01), and exercise had no significant effect on hippocampal NHI (P0.05).
Compared with group ES, NHI in group EL decreased significantly (P0.01), and there was no significant change in EH group (P0.05). Compared with group SS, SL group NHI decreased significantly (P0.01), SH group had no significant changes (P0.05).
Compared with the first resting group, NHI in EH group increased (P0.05), ES and EL group did not change significantly (P0.05), NHI in SH group increased significantly (P0.01), SS and SL group did not change significantly (P0.01).
research conclusion
1. long term low iron diet can cause nutritional iron deficiency in rats.
2. in the low iron diet, long-term exercise aggravates the reduction of iron status in the serum of rats; when the iron content is sufficient, long term exercise leads to Hb, HCT increase, but no change in serum iron; in the high iron diet, the long-term exercise has no effect on the blood iron status.
3. exercise and dietary iron content of two factors have interaction with the female rats, the dietary iron content has an effect on the hippocampal NHI, and the content of NHI in the hippocampus of the diet group of low iron content decreases. In the low iron content diet, the long-term exercise aggravates the decrease of the hippocampal NHI; in the diet iron content and the high iron content diet, the movement of Houhai horse NHI has no significant change.
4. in the diet of low iron content and high iron content diet, the NHI of Qianhai horse decreased obviously, the content of low iron after exercise decreased, while the diet of high iron content recovered to normal level, suggesting that exercise did not affect the NHI of hippocampus in the high iron content diet, and the hippocampus had the mechanism of maintaining iron metabolism.
【学位授予单位】：广州医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R151.41

【参考文献】