十溴联苯醚亚慢性染毒对成年大鼠海马胆碱能系统和γ-氨基丁酸的影响

发布时间：2018-06-03 22:08

  本文选题：十溴联苯醚 + 学习　； 参考：《环境与健康杂志》2015年11期

【摘要】：目的观察十溴联苯醚(BDE-209)对成年大鼠海马胆碱能系统和γ-氨基丁酸(GABA)的影响。方法将64只健康SPF级SD雄性大鼠随机分为4组,溶剂对照(花生油)组和250、500、1 000 mg/kg BDE-209染毒组,每组16只。采用灌胃方式进行染毒,每天1次,连续染毒60 d。采用Morris水迷宫试验检测大鼠的学习和记忆能力;采用化学比色法测定海马组织乙酰胆碱转移酶(Ch AT)、乙酰胆碱酯酶(ACh E)的活力和乙酰胆碱(ACh)的含量,采用免疫组化法检测海马组织GABA的表达情况。结果与溶剂对照组比较,在同一训练时间,各剂量BDE-209染毒组大鼠的逃避潜伏期均延长,跨台次数均减少;海马组织内Ch AT、TCh E活力和ACh含量及CA1区、CA3区和DG内GABA含量均较低,差异有统计学意义(P0.05)。且随着BDE-209染毒剂量的升高,大鼠的逃避潜伏期呈延长趋势,跨台次数呈下降趋势,海马组织内Ch AT、TCh E活力和ACh含量及CA1区、CA3区和DG内GABA表达均呈下降趋势。结论 BDE-209亚慢性染毒导致大鼠学习和记忆能力下降,可能与海马胆碱能系统和GABA表达下降有关。
[Abstract]:Objective To observe the effect of ten brominated diphenyl ether (BDE-209) on the hippocampal cholinergic system and gamma aminobutyric acid (GABA) in adult rats. Methods 64 healthy SPF grade SD male rats were randomly divided into 4 groups, solvent control (peanut oil) group and 250500,1 000 mg/kg BDE-209 group, 16 rats in each group, and 1 times a day, 60 d were continuously poisoned by gavage. The Morris water maze test was used to detect the learning and memory ability of rats; the activity of acetylcholine transferase (Ch AT), the activity of acetylcholinesterase (ACh E) and the content of acetylcholine (ACh) in the hippocampus were measured by chemical colorimetry. The expression of GABA in the hippocampus was detected by immunohistochemistry. The results were compared with that of the solvent control group in the same training. The escape latency of the rats in each dose of BDE-209 was prolonged and the number of cross stage decreased, and the content of Ch AT, TCh E activity, ACh content and CA1 area, CA3 and DG GABA content in the hippocampus were lower, and the difference was statistically significant (P0.05). The number of Ch AT, TCh E activity and ACh content and CA1 area in hippocampus, CA3 region and DG in DG are all decreasing. Conclusion BDE-209 subchronic poisoning leads to the decline of learning and memory ability in rats, which may be related to the decrease of hippocampal cholinergic system and GABA expression.
【作者单位】： 四川医科大学公共卫生学院;四川医科大学病理教研室;四川医科大学;四川医科大学解剖学教研室;
【基金】：四川省教育厅基金(15ZB0151) 四川省科技厅课题(2010JY0128) 四川省卫生厅基金(100207)
【分类号】：R114
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本文编号：1974376