高效液相色谱法同时测定工作场所空气中二甲基甲酰胺和二甲基乙酰胺

发布时间：2018-06-04 18:26

本文选题：二甲基甲酰胺 + 二甲基乙酰胺　；参考：《中国职业医学》2015年01期

【摘要】：目的建立工作场所空气中二甲基甲酰胺(DMF)和二甲基乙酰胺(DMA)的硅胶管采集高效液相色谱测定方法。方法工作场所空气中DMF和DMA用硅胶管采集,纯水解吸,经Eclipse Plus C18液相色谱柱分离,二极管点阵检测器检测,外标法定量。结果 DMF和DMA质量浓度在20.00~400.00 mg/L时,标准曲线线性关系良好,相关系数分别为0.999 7和0.999 9;按采集空气样品3.0 L计算,最低检出浓度分别为0.03和0.05 mg/m3;批内及批间精密度均5.0%;平均加标回收率分别为99.0%~99.6%和99.6%~100.2%;采样效率分别为98.2%~100.0%和97.4%~100.0%;穿透容量分别为10.2和9.9 mg;解吸效率分别为93.0%~96.9%和94.0%~98.5%;样品在常温条件下可保存7 d。结论本方法灵敏度高、精密度好及定性能力强,能更好适应工作场所空气中DMF和DMA检测。
[Abstract]:Objective to establish a high performance liquid chromatography (HPLC) method for the determination of dimethylformamide (DMF) and dimethyl acetamide (DMA) in workplace air. Methods DMF and DMA in workplace air were collected by silica gel tube, desorbed by pure water, separated by Eclipse Plus C18 liquid chromatographic column, detected by diode lattice detector and quantified by external standard method. Results when the mass concentration of DMF and DMA was 20.00 渭 m 400.00 mg/L, the linear relationship of the standard curve was good, the correlation coefficients were 0.999 7 and 0.999 9, respectively. The lowest detectable concentrations were 0.03 and 0.05 mg / m ~ (3), respectively; the precision in and between batches were 5.0; the average recoveries were 99.0 ~ 99.6% and 99.6% respectively; the sampling efficiencies were 98.22% and 97.4%; the penetration volumes were 10.2 and 9.9 mg; the desorption efficiencies were 93.0% and 94.0 ~ 98.5; It can be stored for 7 days under normal temperature. Conclusion this method has the advantages of high sensitivity, good precision and good qualitative ability. It can better adapt to the detection of DMF and DMA in the air of workplace.
【作者单位】：肇庆市疾病预防控制中心;
【基金】：肇庆市科技创新计划项目(2014E2812)
【分类号】：R134.4;O657.72

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