普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理

发布时间：2018-06-05 09:43

本文选题：HepG + 脂质代谢　；参考：《食品科学》2017年17期

【摘要】：以正常培养和油酸诱导培养的人肝癌细胞系(human hepatocellular liver carcinoma cell line,Hep G2)细胞为模型,通过测定普洱茶茶色素对Hep G2细胞内甘油三酯(triglyceride,TG)和总胆固醇(total cholesterol,TC)含量,细胞中脂肪酸合成酶(fatty acid synthase,FAS)、固醇调节元件结合蛋白1c(sterol regulatory element binding protein 1c,SREBP-1c)、三磷酸腺苷结合转运子A1(ATP binding cassette transporter A1,ABCA1)、胆固醇7α-羟化酶(cholesterol 7α-hydroxylase,CYP7A1)的转录水平,磷酸化腺苷酸活化蛋白激酶(phospho-AMPactivated protein kinase,p-AMPK)的蛋白表达水平研究普洱茶茶色素的减肥降脂作用机制。结果显示,普洱茶茶色素能明显降低油酸诱导的Hep G2细胞模型中TG和TC含量,作用程度依赖于普洱茶的作用质量浓度。但对正常培养的Hep G2的TG、TC作用影响不显著。经过普洱茶茶色素作用油酸诱导Hep G2细胞24 h后,能显著下调细胞的FAS和SREBP-1c的m RNA表达水平(P0.05),显著上调ABCA1的转录水平(P0.05),且使CYP7A1的转录水平呈上升趋势,并显著上调p-AMPK蛋白的表达量(P0.05)。因此,普洱茶茶色素可通过调控上述调控因子和酶的表达而改善油酸诱导下Hep G2细胞的脂质代谢水平。
[Abstract]:The normal and oleic acid-induced human hepatocellular liver carcinoma cell lineage Hep G2 (Hep G2) cells were used as models. The contents of triglyceride (TG) and total cholesterol (TC) in Hep G2 cells treated with tea pigment from Pu'er tea were measured. The transcriptional levels of fatty acid synthase (acid synthase), steroid regulatory element binding protein (1c(sterol regulatory element binding protein 1), adenosine triphosphate binding transporter (A1(ATP binding cassette transporter A1) ABCA1, cholesterol 7 伪 -hydroxylase1 (CYP7A1) and fatty acid synthase (acid synthase) were detected. Protein expression of phosphorylated adenylate activated protein kinase phospho-AMPactivated protein kinase- p-AMPK study on the mechanism of reducing fat and reducing fat of tea pigment in Pu'er tea. The results showed that the content of TG and TC in Hep G2 cells induced by oleic acid was significantly decreased by tea pigment, which depended on the action concentration of Pu'er tea. However, the effect of Hep G2 on TC was not significant. After induced by oleic acid for 24 h, the expression level of m RNA of FAS and SREBP-1c in Hep G2 cells was significantly down-regulated, and the transcription level of ABCA1 was upregulated, and the transcription level of CYP7A1 was on the rise. The expression of p-AMPK protein was significantly up-regulated (P 0.05). Therefore, tea pigment can improve the lipid metabolism of Hep G2 cells induced by oleic acid by regulating the expression of these regulatory factors and enzymes.
【作者单位】：天津商业大学生物技术与食品科学学院天津市食品与生物技术重点实验室;云南天士力帝泊洱生物茶集团有限公司;
【基金】：天津市高等学校创新团队建设规划资助项目(TD12-5049) 天津市高校科技发展基金计划项目(20120603) 天津商业大学大学生研究训练计划(SRT)基金项目
【分类号】：R151

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