母源性丙烯酰胺暴露对子代鼠皮质神经元MAP-2与氧化应激的影响

发布时间：2018-06-07 07:24

本文选题：丙烯酰胺 + 神经发育毒性　；参考：《神经解剖学杂志》2017年04期

【摘要】：目的:探讨母源性丙烯酰胺(acrylamide,ACR)暴露对子代鼠大脑皮质神经元MAP-2与氧化应激的影响。方法:SD孕鼠随机分为4组,自怀孕第6 d至怀孕第19 d起对照组和实验组分别灌胃给药给予双蒸水和每日8,16和24 mg/kg ACR溶液。HE和尼氏染色观察子代鼠皮质神经元形态学改变,免疫组织化学和Western Blot检测子代鼠皮质神经元MAP-2的表达,应用试剂盒检测大脑皮质组织中氧化应激指标丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、还原谷胱甘肽(GSH)。结果:(1)MAP-2表达:与对照组相比,实验组中、高剂量组MAP-2表达下降,差异有统计学意义(P0.05),而低剂量组则差异不显著(P0.05)。(2)氧化应激指标变化:与对照组相比,实验组中、高剂量组子代鼠大脑皮质组织中MDA含量显著升高,而T-SOD活力和GSH含量显著下降,差异具有统计学意义(P0.05)。结论:丙烯酰胺致子代鼠神经元损伤的机制可能与其诱导氧化应激损伤有关。
[Abstract]:Aim: to investigate the effects of exposure to maternal acrylamide (ACR) on MAP-2 and oxidative stress in cerebral cortex neurons of offspring rats. Methods Twenty SD pregnant rats were randomly divided into 4 groups. From the sixth day of pregnancy to the 19th day of pregnancy, the control group and the experimental group were given double steamed water and 816 and 24 mg/kg ACR solution respectively. The morphological changes of cortex neurons were observed by HE and Nissl staining. Immunohistochemistry and Western Blot were used to detect the expression of MAP-2 in cortical neurons of offspring rats. The oxidative stress index of malondialdehyde (MDA) in cerebral cortex, total superoxide dismutase (T-SOD), and reduced glutathione (GSH) were detected by kit. Results: compared with the control group, the expression of MAP-2 in the high dose group was significantly lower than that in the control group, but the difference was not significant in the low dose group. The content of MDA in cerebral cortex of offspring of high dose group was significantly increased, while the activity of T-SOD and the content of GSH were decreased significantly. The difference was statistically significant (P 0.05). Conclusion: the mechanism of neuronal injury induced by acrylamide may be related to oxidative stress injury induced by acrylamide.
【作者单位】：肇庆医学高等专科学校;
【基金】：广东省医学科学基金项目(A201618) 广东省中医药局中医药常规科研项目(20162156) 肇庆市科技创新指导项目(2014E184) 肇庆市医学高等专科学校青年科技基金(zqyq16-001)
【分类号】：R114

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