辐射对男性生殖系统及血液系统的影响

发布时间：2018-06-09 19:47

本文选题：X射线 + 外周血　；参考：《苏州大学》2012年硕士论文

【摘要】：目的：采用单细胞凝胶电泳技术检测X射线对人离体外周血中有核细胞的DNA的损伤及损伤修复；探讨不同剂量的X射线对人离体精液DNA及运动性的损伤；采用单细胞凝胶电泳技术检测不同剂量X射线对人离体外周血有核细胞DNA及精子DNA的损伤,评估外周血有核细胞及精子在高剂量X射线照射后DNA的损伤程度。方法：采用正常人的离体外周血,用能量为6MV的X射线分别给予0Gy、2Gy、4Gy、6Gy、8Gy、10Gy的剂量照射,分别在0.5h,lh,2h用单细胞凝胶电泳技术检测全血中有核细胞的彗星率,彗尾长度和尾部DNA含量。采用正常人离体的精液以能量为6MeV的X射线分别给予0Gy、2Gy、4Gy、6Gy、8Gy、10Gy剂量的照射。照射后lh,2h,4h,6h,8h,10h,12h检测精液常规,观察精子活率,精子活力；照射后1h内行单细胞凝胶电泳,观察彗星率,彗尾长度,尾部DNA含量。采用正常人的外周血和采集正常的人精液,以能量为6MV的X射线分别给予0Gy、2Gy、4Gy、6Gy、8Gy、10Gy的剂量照射。照射后1h内行单细胞凝胶电泳检测人离体外周血中有核细胞和人离体精液中精子的彗星率,彗尾长度和尾巴DNA含量。结果： 1.0Gy的对照组彗星率1%,2Gy时彗星率到达27.10%,4Gy时彗星率达到76.32%,6Gy,8Gy,10Gy时彗星率在90%以上,接近100%。2Gy,4Gy时经2h修复彗星率下降具有统计学意义(p0.05)；6Gy、8Gy、10G时彗星率下降无统计学意义(p0.05)。DNA修复在1小时内最快,在1h-2h间修复速率较1h内修复速率减慢。 2.2Gy、4Gy、6Gy、8Gy、10Gy剂量照射组与0Gy对照组相比精子的活率和精子活力在各时间点没有统计学差异(P0.05)；2Gy、4Gy、6Gy、8Gy、10Gy剂量照射组的彗星率分别是47.37±8.32%,92.22±9.025%,100%,100%,100%；与0Gy组(2.10±1.50%)相比彗星率明显升高,具有明显的统计学差异(P0.05)。 3.0Gy对照组人外周血中有核细胞的彗星率和精液中精子的彗星率分别是1.00±0.10%,2.1±1.5%,有统计学差异(p0.05)；2Gy组人离体外周血中有核细胞的彗星率和精液中精子的彗星率分别是27.10±4.71%，47.37±8.32%,有统计学差异(p0.05)；4Gy组人离体外周血中有核细胞的彗星率和精液中精子的彗星率分别是76.32±10.28%，92.22±9.02%，有统计学差异(p0.05)；6Gy组人离体外周血中有核细胞的彗星率和精液中精子的彗星率分别是94.36±3.24%，100%，有统计学差异(p0.05)；8Gy，10Gy组人离体外周血中有核细胞的彗星率和精液中精子的彗星率都分别是100%，100%，无统计学差异。结论：在2Gy以上的X射线照射下全血中有核细胞的DNA均受到不同程度的损伤。6Gy以上DNA损伤接近100%，且修复明显减慢或消失，推测：6Gy的X射线可能是全血细胞所能承受的极限。精子受到不同剂量的X线照射后，精子活率和精子活力没有明显的变化，仍然具有与卵子结合的运动性；精子的DNA受到了严重的损伤。在一定剂量范围内（2-6Gy）精子彗星实验比人离体外周血中单个核细胞彗星实验能更敏感的反应X射线对人体的损伤。
[Abstract]:Objective: to detect the DNA damage and repair of nucleated cells in human peripheral blood by single cell gel electrophoresis (SCGE), and to explore the damage to human semen DNA and exercise induced by different doses of X ray. Single cell gel electrophoresis (SCGE) was used to detect the DNA damage of human peripheral blood nucleated cells (PBMC) and spermatozoa (spermatozoa) induced by different doses of X-ray. To evaluate the degree of DNA damage of nucleated cells and sperm in peripheral blood after high dose X-ray irradiation. Methods: in vitro peripheral blood of normal people were irradiated with 0 Gy 2 Gy 2 Gy 4 Gy 4 Gy 4 Gy 6 Gy 10 Gy of X ray respectively. Comet rate, tail length and tail DNA content of nucleated cells in whole blood were detected by single cell gel electrophoresis (SCGE) at 2 h after 0.5 h. Normal human semen in vitro was irradiated with X-rays of energy of 6 MeV at a dose of 10 Gy of 0 Gy 2 Gym 4 Gym 6 Gym 8 Gy 10 Gy respectively. The sperm motility and sperm motility were observed by single cell gel electrophoresis within 1 hour after irradiation, and the comet rate, tail length and tail DNA content were observed by single cell gel electrophoresis (SCGE) within 1 hour after irradiation. The peripheral blood of normal people and normal human semen were collected and irradiated with X-rays of energy of 6 MV. The doses of 0 Gy ~ 2 Gy ~ (4) Gy ~ (4) Gy ~ (6) Gy ~ (6) Gy ~ (8) Gy ~ (10 Gy) were irradiated respectively. The comet rate of nucleated cells in human peripheral blood and sperm in human semen were detected by single cell gel electrophoresis within 1 hour after irradiation. Results: the comet rate of 1.0Gy control group reached 27.10104Gy and the comet rate reached 76.326Gy / 8Gy / 10Gy, and the comet rate was more than 90% when the comet rate reached 27.1010Gy and 8Gy / 10Gy, respectively. The reduction of comet rate after 2 h repair was statistically significant when the comet rate was close to 100.2 Gy and 4Gy. There was no statistical significance in the reduction of comet rate at the time of p0.05Gy6 Gy1 / 10G, and the highest rate of DNA repair was found within 1 hour. The repair rate among 1h-2h was slower than that in 1 hour. The sperm motility and sperm motility in the 2.2Gy 4Gy 4Gy 6Gy 10Gy irradiation group compared with the 0Gy control group were not significantly different at each time point. The comet rate of the 10 Gy irradiation group was 47.37 卤8.3292.22 卤9.0252510Gy, and was 2.10 卤1.50Gy). Higher than the comet rate, The comets rate of nucleated cells in peripheral blood and the comet rate of spermatozoa in semen were 1.00 卤0.1010 and 2.1 卤1.5, respectively. There was statistical difference in comet rate of nucleated cells in peripheral blood and sperm in semen. The comets rate of human peripheral blood nucleated cells and sperm were 76.32 卤10.28 卤92.22 卤9.02, respectively. There was statistical difference between the two groups (p 0.05Gy) and spermatozoa (P 0.056Gy), respectively. There was statistical difference in the comets rate of nucleated cells in human peripheral blood and spermatozoa in human peripheral blood of the group of p 0.056.Gy. The comet rate of human peripheral blood was significantly higher than that of the control group (p 0.056.Gy), and the rate of sperm was 76.32 卤10.28 卤92.22 卤9.02, respectively. There was a significant difference between the two groups. The comets rate of sperm in the liquid was 94.36 卤3.24g / 100, respectively. There was statistical difference in the comets rate of nucleated cells in human peripheral blood and sperm in human peripheral blood and semen in vitro, respectively. Conclusion: there is no statistical difference between the comet rate of sperm and that of spermatozoa. Conclusion: the rate of X-ray irradiation above 2 Gy is higher than 2 Gy. DNA of nucleated cells in the whole blood was damaged by different degrees. The DNA damage was close to 100 Gy, and the repair slowed down or disappeared obviously. It is speculated that the X-ray of 6 Gy may be the limit of the whole blood cell. After different doses of X-ray irradiation, sperm motility and sperm motility remained unchanged, and sperm DNA was seriously damaged. The comet assay of sperm in a certain dose range is more sensitive than the comet assay of mononuclear cells in human peripheral blood to respond to the damage caused by X-ray.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R144

【参考文献】