不同时期铅暴露对小鼠学习记忆能力及海马组织中β淀粉样蛋白表达的影响

发布时间：2018-06-13 19:18

本文选题：铅 + 小鼠　；参考：《郑州大学学报(医学版)》2015年03期

【摘要】：目的:探讨不同时期铅暴露对小鼠学习记忆能力以及海马组织中β淀粉样蛋白(Aβ)表达的影响。方法:将40只健康雄性仔鼠随机分为生长期暴露组[母鼠饮2 g/L的醋酸铅水溶液经母乳给仔鼠染毒,直至断乳(21d)]、成年期暴露组(出生后第180~240天给予2 g/L的醋酸铅水溶液)、共同暴露组(按照上述方式在生长期及成年期均染铅)以及对照组(饮用去离子水)4组,每组10只。1 a后,分别用Morris水迷宫及ELISA测定各组小鼠的学习记忆能力以及海马组织中Aβ含量。结果:各组小鼠平均逃避潜伏期差异有统计学意义(F时间=396.688,F组间=177.902,P均0.001);各暴露组小鼠穿越平台次数少于对照组(F=30.394,P0.001),生长期暴露组和共同暴露组小鼠穿越平台的次数少于成年期暴露组(P均0.05);各暴露组小鼠海马组织中Aβ含量高于对照组(F=346.032和53.796,P均0.001),且生长期暴露组和共同暴露组Aβ含量高于成年期暴露组(P0.05)。结论:生长期铅暴露造成海马组织中Aβ蓄积更严重,小鼠学习记忆能力的损害也更严重。
[Abstract]:Aim: to investigate the effects of lead exposure on learning and memory ability and the expression of 尾 -amyloid protein A 尾 in hippocampus of mice. Methods: forty healthy male rats were randomly divided into two groups: exposed group [female rats drinking 2 g / L lead acetate solution] was fed with breast milk. The adult exposure group (180 to 240 days after birth was given 2 g / L of lead acetate solution), the co-exposure group (which was exposed to lead in the growth and adult stages according to the above manner) and the control group (10 rats in each group after drinking deionized water). The ability of learning and memory and the content of A 尾 in hippocampus were measured by Morris water maze and Elisa. Results: the average escape latency of each group was significantly different from that of the control group (P < 0.01), and the times of crossing the platform in each exposure group were less than those in the control group (P 0.001), and in the exposed group and co-exposure group, the number of crossing the platform was less than that of the control group (P < 0.05), and the number of mice in the exposure group and the co-exposure group was less than that in the control group. The content of A 尾 in hippocampus of each exposure group was higher than that of control group (P 346.032 and P 0.001), and the content of A 尾 in exposed group and co-exposure group was higher than that in adult exposure group (P 0.05). Conclusion: the accumulation of A 尾 in hippocampal tissue is more serious due to lead exposure in growing stage, and the learning and memory ability of mice is also more serious.
【作者单位】：郑州大学公共卫生学院营养与食品卫生学教研室;河南大学护理学院;河南省疾病预防控制中心健康教育与慢病防治研究所;北京西城区陶然亭社区卫生服务中心;
【基金】：国家自然科学基金资助项目81172716
【分类号】：R114

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