ROS介导的氧化应激在INH诱导的细胞毒性中的作用及槲皮素的干预

发布时间：2018-06-15 13:23

本文选题：槲皮素 + 异烟肼　；参考：《生态毒理学报》2015年03期

【摘要】：为探讨ROS介导的氧化应激在异烟肼(INH)诱导L-02细胞毒性中的作用及槲皮素的干预作用,建立体外培养INH诱导L-02细胞氧化损伤模型,实验分为对照组(A)、INH组(B)、槲皮素低剂量组(C)及槲皮素高剂量组(D)。采用生化分析法检测L-02细胞培养液中天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)的活性;利用荧光探针检测L-02细胞线粒体内活性氧(ROS)水平;应用比色法检测L-02细胞内丙二醛(MDA)、谷胱甘肽(GSH)的含量以及主要抗氧化物酶的活性。结果表明,与对照组相比,INH能显著增加L-02细胞培养液中AST和ALT的活性、细胞线粒体内ROS水平及细胞内MDA的含量(P0.01),并显著减少L-02细胞内GSH的含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性(P0.01)。与INH组比较,槲皮素低剂量组L-02细胞培养液中AST的活性、线粒体内ROS水平及细胞内MDA的含量明显降低(P0.05),而细胞内SOD的活性明显增加(P0.05);高剂量槲皮素能显著降低L-02细胞培养液中AST和ALT的活性、细胞线粒体内ROS水平及细胞内MDA的含量(P0.01),并能显著增高L-02细胞内GSH的含量和主要抗氧化物酶的活性(P0.01)。与槲皮素低剂量组相比,槲皮素高剂量组的保护效应更明显(P0.05)。可见,ROS介导的氧化应激在INH诱导的L-02细胞毒性中发挥了重要作用,且槲皮素对INH诱导的L-02细胞氧化损伤具有保护作用。
[Abstract]:To investigate the role of Ros mediated oxidative stress in the cytotoxicity of L-02 cells induced by isoniazid (INH) and the intervention of quercetin, a model of oxidative damage of L-02 cells induced by INH in vitro was established. The experiment was divided into two groups: the control group, the low dose group, and the high dose group, the control group, the high dose group and the high dose group, respectively. The activities of aspartate aminotransferase (AST) and alanine aminotransferase (alt) in L-02 cell culture medium were detected by biochemical analysis, and the levels of reactive oxygen species (Ros) in mitochondria of L-02 cells were detected by fluorescence probe. The content of malondialdehyde (MDA) and glutathione (GSH) and the activities of main antioxidant enzymes in L-02 cells were detected by colorimetry. The results showed that the activities of AST and alt in L-02 cell culture medium were significantly increased by Inh compared with the control group. The contents of Ros and MDA in mitochondria were significantly decreased in L-02 cells, and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in L-02 cells were significantly decreased (P 0.01). Compared with INH group, the AST activity in L-02 cell culture medium of quercetin low-dose group, The levels of Ros in mitochondria and the content of MDA in cells decreased significantly, while the activities of SOD in cells increased significantly, and the activities of AST and alt in L-02 cells were significantly decreased by high dose quercetin. The level of Ros and the content of MDA in cell mitochondria were significantly increased (P 0.01), and the content of GSH and the activity of main antioxidant enzymes in L-02 cells were significantly increased (P 0.01). The protective effect of high dose quercetin group was more obvious than that of quercetin low dose group. It can be seen that oxidative stress mediated by Ros plays an important role in the cytotoxicity of L-02 cells induced by INH, and quercetin has a protective effect on the oxidative damage of L-02 cells induced by INH.
【作者单位】：佳木斯大学;
【基金】：国家自然科学基金资助项目(81373497) 黑龙江省自然科学基金资助项目(D201248) 佳木斯大学科学技术研究项目(Sjz2012-09)
【分类号】：R114

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