稀土氧化钕粉尘对大鼠肺泡巨噬细胞凋亡及其蛋白水平影响

发布时间：2018-06-16 06:07

本文选题：稀土 + 氧化钕　；参考：《中国职业医学》2015年01期

【摘要】：目的探讨稀土氧化钕(Nd2O3)粉尘对大鼠肺泡巨噬细胞(AM)凋亡及蛋白水平的影响。方法以无特定病原体级健康成年雄性SD大鼠为研究对象,采用非暴露式气管内一次性灌注法建立大鼠肺损伤模型。实验分组和处理方案如下:1实验组大鼠75只,以质量浓度为50.0 g/L的Nd2O3混悬液按100 mg/kg体质量给药剂量进行染尘处理;对照组大鼠50只,予相应的无菌生理氯化钠溶液处理;分别于染尘后第3、7、14、21、28天各处死15和10只;225、50、100、150 mg/kg剂量组大鼠各15只,分别以质量浓度为12.5、25.0、50.0、75.0 g/L的Nd2O3混悬液按25、50、100和150 mg/kg体质量的给药剂量进行染尘处理;对照组大鼠10只,予相应的无菌生理氯化钠溶液处理;于染尘后第28天处死。收集大鼠支气管肺泡灌洗液,分离AM,采用流式细胞仪测定AM凋亡率,酶联免疫吸附实验测定AM蛋白水平。结果肺组织病理学观察显示染尘大鼠肺损伤模型建立成功。AM凋亡率和蛋白水平在染尘处理与观察时间的交互效应均有统计学意义(P0.01);实验组大鼠各时间点AM凋亡率和蛋白水平分别高于相应时间点的对照组(P0.05);实验组大鼠染尘后随着观察时间的延长AM凋亡率和蛋白水平总体上均呈现先升高后下降再升高的趋势,表现为与第3天比较,上述2个指标均在第7天升高(P0.05),在第14天下降至最低值(P0.05),在第21和28天出现升高(P0.05)。不同剂量组间大鼠AM凋亡率随染尘剂量的增加而增加(P0.05),呈剂量-效应关系。25、50、100和150 mg/kg剂量组大鼠AM蛋白水平均高于对照组(P0.05),50、100和150 mg/kg剂量组大鼠AM蛋白水平均高于25 mg/kg剂量组(P0.05)。结论稀土Nd2O3粉尘可致染毒大鼠肺泡AM凋亡和蛋白水平的改变。
[Abstract]:Objective to investigate the effect of ND _ 2O _ 3 dust on apoptosis and protein level of alveolar macrophages in rats. Methods the model of lung injury was established in adult male SD rats without specific pathogens by using unexposed intratracheal instillation. The experimental group and treatment plan were as follows: 75 rats in the experiment group were treated with ND _ 2O _ 3 suspension of 50.0 g / L mass concentration according to the dose of 100 mg/kg body weight, 50 rats in the control group were treated with corresponding aseptic physiological sodium chloride solution, and 50 rats in the control group were treated with aseptic physiological sodium chloride solution. On the 28th day after exposure to dust, 15 rats were killed in each group and 10 rats were killed in the dose group of 225550100150 mg/kg. The rats were treated with Nd2O3 suspension with a concentration of 12.5g / L 25.0g / L or 75.0 g / L respectively according to the dosages of 2550100 and 150 mg/kg body weight, 10 rats in the control group were treated with Dust Dust at the dose of 2550100 and 150 mg/kg respectively, and 10 rats in the control group were treated with Dust 2O 3 suspension with a mass concentration of 12.5g / L, 25.0g / L and 75.0 g / L respectively. The rats were treated with corresponding aseptic physiological sodium chloride solution and killed on the 28th day after exposure to dust. The bronchoalveolar lavage fluid (BALF) of rats was collected and AMAM was isolated. The apoptosis rate of AM was measured by flow cytometry and the level of AM protein was determined by enzyme-linked immunosorbent assay (Elisa). Results the lung histopathological observation showed that the rate of apoptosis and the level of protein in the dust-exposed rat lung injury model were significantly different between the time of exposure and the observation time, and the apoptotic rate of AM at different time points in the experimental group was significantly higher than that in the control group. The apoptotic rate and protein level of AM in the experimental group increased at first and then decreased and then increased with the prolongation of observation time, and the protein levels were higher than those of the control group at the corresponding time points. Compared with the 3rd day, the above two indexes all increased on the 7th day, decreased to the lowest value on the 14th day, and increased on the 21st and 28th days. The apoptotic rate of AM increased with the increase of the dose of dust among different dose groups, and the level of AM protein in the dose-response group of 2550100 and 150 mg/kg was higher than that of the control group. The level of AM protein was higher than that of the control group at the dose of P0.05, 50 and 150 mg/kg, respectively, and the level of AM protein was higher than that in the group of 25 mg/kg. Conclusion rare earth ND _ 2O _ 3 dust can induce apoptosis and protein level of alveolar AM in rats.
【作者单位】：包头医学院公共卫生学院;
【基金】：国家自然科学基金(81260426) 内蒙古自然科学基金(2012MS1102)
【分类号】：R135.2

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