二氧化钛纳米材料对氧化应激状态大鼠肾脏的毒性
本文选题:纳米二氧化钛 + 四氧嘧啶 ; 参考:《西安交通大学学报(医学版)》2015年04期
【摘要】:目的研究二氧化钛(TiO2)纳米材料对正常及氧化应激SD大鼠肾组织的不良影响。方法通过肌肉注射四氧嘧啶,构建氧化应激模型,并分为四氧嘧啶处理组(OS组)、纳米TiO2处理组(NM组)及四氧嘧啶与纳米TiO2共处理组(OS-NM组),后两组分别采用一次性腹腔注射0.5、5和50mg/kg体质量纳米TiO2的方式进行染毒,另设正常对照组。检测氧化应激指标、尿素氮(BUN)水平并制备肾组织病理切片。结果注射5mg/kg体质量纳米TiO2时,OS-NM组大鼠O2-·和超氧化物歧化酶(SOD)较OS组和NM组增高,差异均有统计学意义(P0.05);当注射50mg/kg体质量纳米TiO2时,与OS组及NM组分别比较,OS-NM组大鼠的O2-·、谷胱甘肽(GSH)和SOD水平变化差异均极显著(P0.01);且OS组与NM组相比较,O2-·和GSH的变化有统计学意义(P0.05)。与染毒后的正常大鼠相比,纳米TiO2导致氧化应激大鼠BUN水平的异常升高(P0.01),并引起肾脏出现肾小球体积增大、细胞数目增多、肾小管上皮细胞水肿。方差分析表明四氧嘧啶与纳米TiO2在导致O2-·水平升高的过程中存在协同作用。结论 TiO2纳米材料对正常及氧化应激大鼠肾脏均有损伤,且四氧嘧啶与纳米TiO2协同作用能诱发更为严重的氧化应激状态从而加重对肾脏的损伤,这为患者安全使用纳米材料提供了一定的实验依据。
[Abstract]:Objective to study the adverse effects of titanium dioxide (TiO2) nanomaterials on the renal tissue of normal and oxidative stress SD rats. Methods the oxidative stress model was constructed by intramuscular injection of four oxouracil, and divided into four alloxan treatment group (group OS), nanoscale TiO2 treatment group (NM group) and four oximidine and nano TiO2 co processing group (group OS-NM), and the latter two groups were used respectively. One time intraperitoneal injection of 0.5,5 and 50mg/kg body mass nanoscale TiO2 was carried out, and the normal control group was set up. The oxidative stress index, the urea nitrogen (BUN) level and the renal tissue pathological section were detected. The results showed that the O2- and superoxide dismutase (SOD) of the OS-NM group was higher than that of the OS and NM groups when the mass of 5mg/kg body was injected into the nano TiO2. Statistical significance (P0.05); when the mass of 50mg/kg body was injected with nano TiO2, the difference of O2-, O2-, glutathione (GSH) and SOD in group OS-NM was very significant (P0.01) compared with group OS and NM group, and the change of OS group was statistically significant compared with that of NM group. The abnormal increase of BUN level in oxidative stress rats (P0.01) and the increase of glomerular volume, number of cells and edema of renal tubular epithelial cells were caused in the kidney. The analysis of variance analysis showed that four oxalacil and nano TiO2 have synergistic effect in the process of increasing the level of O2-. The TiO2 nanomaterials on normal and oxidative stress rats' kidneys The synergism between four pyrimidine and nanoscale TiO2 can induce more serious oxidative stress and aggravate the damage to the kidney, which provides a certain experimental basis for the safe use of nanomaterials.
【作者单位】: 西安医学院基础医学部基础医学研究所;西安交通大学医学部第一附属医院麻醉科;
【基金】:国家自然科学基金青年项目(No.81301308,No.81301040) 陕西省自然科学基础研究计划青年项目(No.2014JQ4158) 陕西省青年科技新星项目(No.2014KJXX-76)~~
【分类号】:R114
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